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A new initiative in micropropagation: airborne bacterial volatiles modulate organogenesis and antioxidant activity in tobacco (Nicotiana tabacum L.) callus

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Abstract

The present study aims to analyze the effect of bacterial volatiles on tobacco callus organogenesis. A glass prototype was designed in the shape of the letter ‘H’ such that plants and bacteria could be grown in physically separated compartments while volatile compounds could still be exchanged. Volatiles from a beneficial bacterium, Bacillus badius M12, which showed growth promotion in Sesamum indicum (sesame), also induced organogenesis in tobacco callus. Volatiles from B. badius M12 appeared to maintain tobacco callus in a green and viable state during the prolonged, 45 d, sealed incubation. This apparent antioxidant effect was investigated by exposing freshly cut cylinders of apple flesh, a tissue noted for its ready tendency to oxidize, to bacterial volatiles. The expression of superoxide dismutase (SOD), a key antioxidant enzyme, was higher in callus exposed to bacterial volatiles, confirming that they enhanced antioxidant activity. Exposure of apple tissues to volatile compounds from B. badius M12 and a well-known growth-promoting bacterial volatile, 2,3-butanediol, led to increased antioxidant activity. We conclude that plant growth-promoting bacterial volatiles were useful in tissue organogenesis and increased antioxidant activity.

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Acknowledgments

This research work was financially supported by the Ministry of Environment and Forests (MoEF), Govt. of India. We would like to thank Dr. T. Boopathi for his help during experiments and manuscript preparation. We are thankful to Dr. N. Jayabalan, Department of Plant Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India and his research students for providing plant seeds and for helping during callus preparation. Language editing by Dr. Carla A. Spence, Department of Plant and Soil Sciences, University of Delaware, Newark, Delaware, USA has greatly improvised our manuscript.

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  1. Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India

    Selvaraj Gopinath, Karuppiah Senthil Kumaran & Muthuraman Sundararaman

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  1. Selvaraj Gopinath
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  2. Karuppiah Senthil Kumaran
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  3. Muthuraman Sundararaman
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Correspondence to Muthuraman Sundararaman.

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Gopinath, S., Kumaran, K.S. & Sundararaman, M. A new initiative in micropropagation: airborne bacterial volatiles modulate organogenesis and antioxidant activity in tobacco (Nicotiana tabacum L.) callus. In Vitro Cell.Dev.Biol.-Plant 51, 514–523 (2015). https://doi.org/10.1007/s11627-015-9717-6

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