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Callus induction and plant regeneration from anthers of Dendrocalamus latiflorus Munro

  • Anther culture/haploids
  • Published:
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Abstract

Bamboo varieties are very difficult to improve by traditional breeding methods. Here, we established an efficient plant-regeneration system for Dendrocalamus latiflorus (tropical giant bamboo) by anther culture. Culture conditions, especially the plant growth regulators required for callus induction and shoot differentiation, were optimized by orthogonal design. M8 medium supplemented with 5.37 μΜ α-naphthaleneacetic acid (NAA), 1.33 μM N 6-benzyladenine (BA), 110.17 μM phenylacetic acid (PAA), and a pretreatment time of 3 d produced the highest rate (5.08 ± 0.61%) of callus induction. The maximum shoot differentiation rate reached 28.3 ± 4.29% in M8 medium supplemented with 2.32 μM kinetin (KT), 8.89 μM BA, 1.08 μM NAA, and 110.17 μM PAA. The results of the ploidy level test showed that most of the regenerated plants were dodecaploid (96/100), a few were hexaploid (3/100), and one was triploid (1/100). The average chlorophyll content of dodecaploid lines was significantly higher than that of hexaploid lines. The present study provides an innovative method for bamboo ploidy breeding and a useful method for genetic improvement.

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Acknowledgments

The authors thank Professor Zongxiu Sun at the National Key lab of Rice Biology, Chinese National Rice Research Institute for technical advice. This work was supported by National Natural Science Foundation of China (31200508), Zhejiang Public Technology Research (2012C22099), Science Foundation of Zhejiang province (2010C12010), and National Non-profit Research Institute of CAF (CAFYBB2010003-1).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China

    Guirong Qiao, Haiying Li, Mingying Liu, Jing Jiang, Ling Zhang & Renying Zhuo

  2. Key Laboratory of Tree Genomics, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang, 311400, China

    Guirong Qiao, Haiying Li, Mingying Liu, Jing Jiang & Renying Zhuo

  3. The Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing, 100091, China

    Yafang Yin

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  1. Guirong Qiao
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  2. Haiying Li
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  4. Jing Jiang
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  5. Yafang Yin
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  6. Ling Zhang
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  7. Renying Zhuo
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Correspondence to Renying Zhuo.

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Editor: J. Forster

G. Qiao and H. Li contributed equally to this work.

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Qiao, G., Li, H., Liu, M. et al. Callus induction and plant regeneration from anthers of Dendrocalamus latiflorus Munro. In Vitro Cell.Dev.Biol.-Plant 49, 375–382 (2013). https://doi.org/10.1007/s11627-013-9498-8

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  • DOI: https://doi.org/10.1007/s11627-013-9498-8

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