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Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos

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Abstract

Recalcitrance to tissue culture is observed in some genotypes of Brassica napus. Several studies have confirmed that Pluronic F-68 has growth-promoting effects on numerous tissue types. This work investigated the effect of the non-ionic surfactant Pluronic F-68 at four concentrations (0.1%, 0.25%, 0.5%, and 1% (w/v)) on the responsiveness of recalcitrant B. napus lines to tissue culture. Microspores from seven populations of B. napus were cultured on Nitsch and Nitsch medium with this compound. The embryos obtained were plated on solid B5 medium supplemented with zeatin for shoot induction. Pluronic F-68 had a highly significant effect on the proportion of shoot regeneration (P < 0.05) in some of the recalcitrant populations. However, no strong dose–response effect was observed. The estimated probability of a shoot occurring in the absence of Pluronic F-68 ranged from 0.04 to 0.31 depending on the genotype, while in the presence of Pluronic F-68, it ranged from 0.07 to 0.53, respectively.

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Acknowledgments

The authors thank Dr Jennifer Smith from Plain Statistics Pty Ltd, Australia, for the statistical analysis of the data, and Mrs Shirley-Ann Cawood from the Victorian Department of Primary Industries, Horsham, Australia, for technical assistance.

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Authors and Affiliations

  1. Department of Primary Industries, Biosciences Research Division, Brassica Germplasm Improvement Group, Private Bag 260, Horsham, VIC, 3401, Australia

    Denise M. Barbulescu & Philip A. Salisbury

  2. AgriProducts, Viterra Ltd, Private Bag 260, Horsham, VIC, 3401, Australia

    Wayne A. Burton

  3. Melbourne School of Land and Environment, University of Melbourne, Melbourne, 3010, Australia

    Philip A. Salisbury

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  1. Denise M. Barbulescu
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  2. Wayne A. Burton
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  3. Philip A. Salisbury
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Correspondence to Denise M. Barbulescu.

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Editor: N.J. Taylor

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Barbulescu, D.M., Burton, W.A. & Salisbury, P.A. Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos. In Vitro Cell.Dev.Biol.-Plant 47, 282–288 (2011). https://doi.org/10.1007/s11627-011-9353-8

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