Skip to main content
SpringerLink
Log in

Impact of colchicine application during callus induction and shoot regeneration on micropropagation and polyploidisation rates in two Miscanthus species

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Grasses from the genus Miscanthus have several characteristics that make them very favourable crops for efficient, low input, multifunctional and environmentally friendly biomass production. This study is aimed to improve a polyploidisation method to effectively induce polyploids in Miscanthus sinensis and Miscanthus x giganteus. Colchicine was applied for 2, 4 or 7 d in micropropagation systems using inflorescence segments at two different points: during callus induction (313 and 626 μM colchicine) and during shoot regeneration from callus (313 μM colchicine). Among the tested combinations, the most effective (up to 40%) was the 4-d colchicine treatment of a shoot-forming callus cultured 4 d before the experiment on regeneration medium under light conditions. In vitro colchicine treatment during callus induction and during shoot regeneration from callus resulted in no chimeric polyploids as well as a very low number of albinos (2.5%). Additionally, some combinations using colchicine did not significantly reduce the rates of micropropagation effectiveness. The obtained material is promising for the creation of new high-biomass-yielding forms in the Miscanthus genus. In all genotypes tested, chromosome doubling significantly increased pollen stainability. According to preliminary results, induced tetraploids are fertile and useful in hybrid production. Leaves of polyploid forms of two genotypes demonstrated significantly greater width in comparison to the controls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.

Similar content being viewed by others

References

  • Askew M. F.; Holmes C. A. The potential for biomass and energy crops in agriculture in Europe, in land use, policy and rural economy terms. Biomass and energy crops II. Asp Appl Biol 65: 365–374; 2001.

    Google Scholar 

  • Barandalla L.; Ritter E.; De Galarreta J. I. R. Oryzalin treatment of potato diploids yields tetraploid and chimeric plants from which euploids could be derived by callus induction. Potato Res 49: 143–154; 2006.

    Article  CAS  Google Scholar 

  • Barnabás B.; Obert B.; Kovács G. Colchicine, an efficient genome-doubling agent for maize (Zea mays L.) microspores cultured in anthero. Plant Cell Rep 18: 858–862; 1999.

    Article  Google Scholar 

  • Chakraborti S. P.; Vijayan K.; Roy B. N.; Qadri S. M. H. In vitro induction of tetraploidy in mulberry (Morus alba L.). Plant Cell Rep 17: 799–803; 1998.

    Article  CAS  Google Scholar 

  • deWet J. M. J. Origins of polyploids. In: Lewis W. H. (ed) Polyploidy: biological relevance. Plenum Press, New York, pp 3–16; 1980.

    Google Scholar 

  • Dhawan O. P.; Lavania U. C. Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87: 81–89; 1996.

    Article  CAS  Google Scholar 

  • Eeckhaut T. G. R.; Werbouck S. P. O.; Leus L. W. H.; Van Bockstaele E. J.; Deberrgh P. C. Chemically induced polyploidization in Spathiphyllum wallisii Regel through somatic embryogenesis. Plant Cell Tiss Organ Cult 78: 241–246; 2004.

    Article  CAS  Google Scholar 

  • Gao S. L.; Zhu D. N.; Cai Z. H.; Xu D. R. Autotetraploid plants from colchicine-treated bud culture of Salvia multiorrhiza Beg. Plant Cell Tissue Organ Cult 47: 73–77; 1996.

    Article  CAS  Google Scholar 

  • GÅ‚owacka K.; Jeżowski S.; Kaczmarek Z. Polyploidization of Miscanthus sinensis and Miscanthus x giganteus by plant colchicine treatment. Ind Crops and Prod 30: 444–446; 2009.

    Article  Google Scholar 

  • Gomez K. A.; Gomez A. A. Statistical procedures for agricultural research. 2nd ed. J. W, New York; 1984.

    Google Scholar 

  • Greef J. M.; Deuter M. Syntaxonomy of Miscanthus x giganteus GREEF et DEU. Angew Bot 67: 87–90; 1993.

    Google Scholar 

  • Gu X. F.; Yang A. F.; Meng H.; Zhang J. R. In vitro induction of tetraploid plants from diploid Zizyphus jujyba Mill. cv. Zhanhua. Plant Cell Rep 24: 671–676; 2005.

    Article  CAS  PubMed  Google Scholar 

  • Holme I. B.; Petersen K. K. Callus induction and plant regeneration from different types of Miscanthus × ogiformis Honda ‘Giganteus’. Plant Cell Tiss Org Cult 45: 43–52; 1996.

    Article  Google Scholar 

  • Lewandowski I. Micropropagation of Miscanthus x giganteus. In: Bajaj Y. P. S. (ed) Biotechnology in agriculture and forestry, Vol 39. High-Tech and Micropropagation V. Springer, New York, pp 239–255; 1997.

    Google Scholar 

  • Lewandowski I.; Schmidt U. Nitrogen, energy and land use efficiencies of Miscanthus, reed canary grass and triticale as determined by the boundary line approach. Agr, Ecosyst Environ 112: 335–346; 2006.

    Article  Google Scholar 

  • Linde-Laursen I. B. Cytogenetic analysis of Miscanthus ‘Giganteus’, an interspecific hybrid. Hereditas 119: 297–300; 1993.

    Article  Google Scholar 

  • Liu G.; Li Z.; Bao M. Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology. Euphytica 157: 145–154; 2007.

    Article  Google Scholar 

  • Lu Ch; Bridgen M. P. Chromosome doubling and fertility study of Alstroemeria aurea × A. caryophyllaea. Euphytica 94: 75–81; 1997.

    Article  Google Scholar 

  • Luo Y. W.; Yen X. C.; Zhang G. Y.; Liang G. H. Agronomic traits and chromosome behaviour of autotetraploid sorghums. Plant Breeding 109: 46–53; 1992.

    Article  Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473–497; 1962.

    Article  CAS  Google Scholar 

  • Nimura M.; Kato J.; Horaguchi H.; Mii M.; Sakai K.; Katoh T. Induction of fertile amphidiploids by artificial chromosome-doubling in interspecific hybrid between Dianthus caryophyllus L. and D. japonicus. Thunb Breed Sci 56: 303–310; 2006.

    Article  Google Scholar 

  • Petersen K. K. Callus induction and plant regeneration in Miscanthus x ogiformis Honda ‘Giganteus’ as influenced by benzyladenine. Plant Cell Tiss Organ Cult 49: 137–140; 1997.

    Article  CAS  Google Scholar 

  • Petersen K. K.; Hagberg P.; Kristiansen K. In vitro chromosome doubling of Miscanthus sinensis. Plant Breeding 121: 445–450; 2002.

    Article  Google Scholar 

  • Petersen K. K.; Hagberg P.; Kristiansen K. Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tiss Organ Cult 73: 137–146; 2003.

    Article  Google Scholar 

  • Predieri S. Mutation induced and tissue culture in improving fruits. Plant Cell Tiss Organ Cult 64: 185–210; 2001.

    Article  CAS  Google Scholar 

  • Rey H. Y.; Sansberro P. A.; Collavino M. M.; Daviña J. R.; Gonzàlez A. M.; Mroginski L. A. Colchicine, trifluralin, and oryzaliny promoted development of somatic embryos in Ilex peraguariensis (Aquifoliaceae). Euphytica 123: 49–56; 2002.

    Article  CAS  Google Scholar 

  • Roy A. T.; Leggett G.; Koutoulis A. In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Rep 20: 489–495; 2001.

    Article  CAS  Google Scholar 

  • Shao J.; Chen Ch; Deng X. In vitro induction of tetraploid in pomegranate (Punica granatum). Plant Cell Tiss Organ Cult 75: 241–246; 2003.

    Article  CAS  Google Scholar 

  • Song P.; Kang W.; Peffley E. B. Chromosome doubling of Allium fistulosum × A. cepa interspecific F1 hybrids through colchicine treatment of regenerating callus. Euphytica 93: 257–262; 1997.

    Article  Google Scholar 

  • Thao N. T. P.; Ureshino K.; Miyajima I.; Ozaki Y.; Okubo H. Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss Organ Cult 72: 19–25; 2003.

    Article  CAS  Google Scholar 

  • Urwin N. A. R.; Horsnell J.; Moon T. Generation and characterization of colchicine-induced autotetraploid Lavandula angustifolia. Euphytica 156: 257–266; 2007.

    Article  Google Scholar 

  • Väinölä A. Polyploidization and Early screening of Rhododendron hybrids. Euphytica 112: 239–244; 2000.

    Article  Google Scholar 

  • Warner D. A.; Ku M. S. B.; Edwards G. E. Photosynthesis, leaf anatomy, and cellular constituents in polyploid C4 grass Panicum virgatum. Plant Physiol 84: 461–466; 1987.

    Article  CAS  PubMed  Google Scholar 

  • Wu J.-H.; Mooney P. Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine. Cell Tiss Organ Cult 70: 99–104; 2002.

    Article  CAS  Google Scholar 

  • Yang X. M.; Cao Z. Y.; An L. Z.; Wang Y. M.; Fang X. W. In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifer L.). Euphytica 152: 217–224; 2006.

    Article  Google Scholar 

  • Zhang Z.; Dai H.; Xiao M.; Liu X. In vitro induction of tetraploids in Phlox subulata L. Euphytica 159: 59–65; 2008.

    Article  Google Scholar 

  • Zlesak D. C.; Thill Ch A; Anderson N. O. Trifluralin-mediated polyploidization of Rosa chinensis minima (Sims) Voss seedlings. Euphytica 141: 281–290; 2005.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479, Poznań, Poland

    Katarzyna Głowacka, Stanisław Jeżowski & Zygmunt Kaczmarek

Authors
  1. Katarzyna GÅ‚owacka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stanisław Jeżowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zygmunt Kaczmarek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Katarzyna GÅ‚owacka.

Additional information

Editor: N. Taylor

Rights and permissions

Reprints and permissions

About this article

Cite this article

Głowacka, K., Jeżowski, S. & Kaczmarek, Z. Impact of colchicine application during callus induction and shoot regeneration on micropropagation and polyploidisation rates in two Miscanthus species. In Vitro Cell.Dev.Biol.-Plant 46, 161–171 (2010). https://doi.org/10.1007/s11627-010-9282-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-010-9282-y

Keywords

Search

Navigation