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In vitro plant regeneration from quality protein maize (QPM)

  • Developmental Biology/Morphogenesis..
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Abstract

Breeding efforts to obtain more nutritious maize materials aimed at alleviating dietary deficiencies in developing countries have resulted in an improved maize germplasm known as quality protein maize (QPM). Quality protein maize has higher contents of tryptophan, lysine, and leucine than common maize, but suffers from some major agronomic drawbacks found in common inbred maize lines, such as susceptibility to insect pests and fungal and bacterial diseases and herbicide sensitivity. The development of a reproducible and efficient protocol for tissue culture of QPM is expected to solve some of these deficiencies. In this work, we have evaluated different formulations for in vitro induction of morphogenic responses in three QPM lines developed by the International Maize and Wheat Improvement Center (CIMMYT): CML (CIMMYT maize line)-145, CML-176, and CML-186. Only CML-176 and CML-186 have proven to be responsive to the in vitro conditions considered in this work, with CML-176 showing the highest efficiency in regenerable callus formation and growth. N6C1 medium was found to be efficient for in vitro culture of QPM, whereas no plants could be regenerated by using MPC medium. From CML-176 embyogenic calli cultured on N6C1 medium, we were able to regenerate up to 0.3 plants per 500 mg fresh weight (FW) callus. Further modifications in this experimental protocol, including the replacement of 3,6-dichloro-o-anisic acid with 2,4-dichlorophenoxyacetic acid and modification of the N6C1 vitamin balance, significantly increased the regeneration response of the induced calli, with up to 16.8 and 9.3 plants recovered per 500 mg FW callus for CML-176 and CML-186, respectively.

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Acknowledgements

This research was supported by funds from CONACyT-SAGARPA (project no. 2004-C01-17), Fondos Mixtos CONACyT-Guanajuato (project no. 06-24-A-033), and CONCyTEG (project no. 04-24-K117-042).

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Authors and Affiliations

  1. Unidad de Biotecnología, Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales y Agropecuarias, Km 6.5 Carretera Celaya-San Miguel de Allende, Celaya, Guanajuato, 38110, Mexico

    Gerardo Armando Aguado-Santacruz & Blanca Moreno-Gómez

  2. Postgrado en Botánica, Colegio de Postgraduados, km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Edo, Mexico, 56230, Mexico

    Edmundo García-Moya

  3. Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales y Agropecuarias, Km 6.5 Carretera Celaya-San Miguel de Allende, Celaya, 38110, Mexico

    José Luis Aguilar-Acuña & Ernesto Solís-Moya

  4. Programa de Mejoramiento Genético de Maíz, Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales y Agropecuarias, Km 6.5 Carretera Celaya-San Miguel de Allende, Celaya, 38110, Mexico

    Ernesto Ricardo Preciado-Ortiz

  5. Departamento de Biología Molecular, Instituto Potosino de Investigacíon Científica y Tecnológica, A.C., Camino a la Presa San José 2055, Lomas 4a Sección, San Luís Potosí, S.L.P. 78216, México

    Juan Francisco Jiménez-Bremont

  6. Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Avenida Universidad s/n, Colonia Universidad, Chihuahua, Chih, 31170, Mexico

    Quintín Rascón-Cruz

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  1. Gerardo Armando Aguado-Santacruz
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  2. Edmundo García-Moya
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  3. José Luis Aguilar-Acuña
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  4. Blanca Moreno-Gómez
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  5. Ernesto Solís-Moya
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  6. Ernesto Ricardo Preciado-Ortiz
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  7. Juan Francisco Jiménez-Bremont
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  8. Quintín Rascón-Cruz
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Correspondence to Gerardo Armando Aguado-Santacruz.

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Editor: P. Ozias-Akins

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Aguado-Santacruz, G.A., García-Moya, E., Aguilar-Acuña, J.L. et al. In vitro plant regeneration from quality protein maize (QPM). In Vitro Cell.Dev.Biol.-Plant 43, 215–224 (2007). https://doi.org/10.1007/s11627-007-9042-9

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