Skip to main content
SpringerLink
Log in

Identification of a factor that complementarily inhibits somatic embryogenesis with vanillyl benzyl ether in Japanese larch

  • Developmental Biology/Morphogenesis
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

In Japanese larch (Larix leptolepis Gordon), a well-developed suspensor forms during somatic embryogenesis. The suspensor is the essential tissue for development of the embryo proper. In high-cell-density culture, the embryogenic cells proliferate, but no somatic embryos form because suspensor development is suppressed. Previously, we identified vanillyl benzyl ether (VBE) as a novel factor suppressing suspensor development from the high-cell-density conditioned medium (HCM), but the inhibitory effect of VBE was weaker than that of HCM added. Therefore, this study attempted to identify another inhibitory factor in the culture medium. Induction of somatic embryos was performed in a medium containing both VBE and a fraction of each chromatogram extracted from the culture medium. Results of the bioassay showed that a fraction had strong inhibitory activity with VBE, but weak activity without it. By physicochemical analyses of the fraction, 4-[(phenylmethoxy)methyl]phenol was identified as an inhibitory factor of larch somatic embryogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.

Similar content being viewed by others

References

  • Bonga, J. M.; Klimaszewska, K.; Lelu, M. A.; von Aderkas, P. Somatic embryogenesis in Larix. In: Jain, S. M.; Gupta, P. K.; Newton, R. J., eds. Somatic Embryogenesis in Woody Plants, vol. 3. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1995:315–339.

    Google Scholar 

  • Borkird, C.; Choi, J. H.; Jin, Z. H.; Franz, G.; Hatzopoulos, P.; Chorneau, R.; Bonas, U.; Pelegri, F.; Sung, Z.R. Developmental regulation of embryogenic genes in plants. Proc. Natl. Acad. Sci. U S A 85:6399–6403; 1988.

    Article  PubMed  CAS  Google Scholar 

  • Campbell, R. A.; Durzan, D. J. Induction of multiple buds and needles in tissue cultures of Picea glauca. Can. J. Bot. 53:1652–1657; 1975.

    Article  CAS  Google Scholar 

  • Ciavatta, V. T.; Morillon, R.; Pullman, G. S.; Chrispeels, M. J.; Cairney, J. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine. Plant Physiol. 127:1556–1567; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Dixon, R. A.; Paiva, N. L. Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097; 1995.

    Article  PubMed  CAS  Google Scholar 

  • Igasaki, T., Akashi, N., Ujino-Ihara, T., Matsubayashi, Y., Sakagami, Y., Shinohara, K. Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica. Plant Cell Physiol. 44: 1412–1416; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Kiyosue, T.; Yamaguchi-Shinozaki, K.; Shinozaki, K.; Higashi, K.; Satoh, S.; Kamada, H.; Harada, H. Isolation and characterization of a cDNA that encodes ECP31, an embryogenic-cell protein from carrot. Plant Mol. Biol. 19:239–249; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Kiyosue, T.; Yamaguchi-Shinozaki, K.; Shinozaki, K.; Kamada, H.; Harada, H. cDNA cloning of ECP40, an embryogenic-cell protein in carrot, and its expression during somatic and zygotic embryogenesis. Plant Mol. Biol. 21:1053–1068; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi, T.; Eun, C.; Hanai, H.; Matsubayashi, Y.; Sakagami, Y.; Kamada, H. Phytosulfokine-α, a peptide growth factor, stimulates somatic embryogenesis in carrot. J. Exp. Bot. 50: 1123–1128; 1999.

    Article  Google Scholar 

  • Matsubayashi, Y.; Sakagami, Y. Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc. Natl. Acad. Sci. U S A 93:7623–7627; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Meinke, D. W. Perspectives on genetic analysis of plant morphogenesis. Plant Cell 3:857–866; 1991.

    Article  PubMed  CAS  Google Scholar 

  • Ogita, S.; Kubo, T.; Fushitani, M. Anatomical characteristics in early embryogenesis from embryo of Larix leptolepis. For. Res. Environ. 35:45–51; 1997.

    Google Scholar 

  • Ogita, S.; Sasamoto, H.; Kubo, T. Maturation and plant recovery from embryogenic cells of Japanese larch: effect of abscisic acid in relation to their morphology. J. For. Res. 4:241–244; 1999.

    CAS  Google Scholar 

  • Ogita, S.; Sasamoto, H.; Kubo, T. Control of the development of somatic embryo of Japanese conifers by the density of embryogenic cells in liquid culture. In: Kubota, C.; Chun, C., eds. Transplant Production in the 21st Century. Dordrecht, The Netherlands: Kluwer Academic Publishers; 2000:209–214.

    Google Scholar 

  • Schwartz, B. W.; Daniel, S.; Vernon, M.; Meinke, D. W. Development of the suspensor: differentiation, communication, and programmed cell death during plant embryogenesis. In: Larkins, B. A.; Vasil, I. K., eds. Cellular and Molecular Biology of Plant Seed Development. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1997:53–72.

    Google Scholar 

  • Shaari, K.; Waterman, P. G. Glucosides of 2,5-dihydroxybenzyl alcohol from Homalium longifolium. Phytochemistry 39:1415–1421; 1995.

    Article  CAS  Google Scholar 

  • Smertenko, A. P.; Bozhkov, P. V.; Filonova, L. H.; Hussey, P. J. Re-organisation of the cytoskeleton during developmental programmed cell death. Plant J. 33:813–824; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Umehara, M.; Ogita, S.; Sasamoto, H.; Kamada, H. Inhibitory factor(s) of somatic embryogenesis regulated suspensor differentiation in suspension culture of Japanese Larch (Larix leptolepis GORDON). Plant Biotechnol. 21:87–94; 2004a.

    CAS  Google Scholar 

  • Umehara, M.; Ogita, S.; Sasamoto, H.; Kamada, H. An in vitro culture system used to investigate possible interactions between the embryo proper and the suspensor in embryogenesis in Japanese Larch (Larix leptolepis GORDON). Plant Biotechnol. 21:169–171; 2004b.

    Google Scholar 

  • Umehara, M.; Ogita, S.; Sasamoto, H.; Koshino, H.; Asami, T.; Fujioka, S.; Yoshida, S.; Kamada, H. Identification of a novel factor, vanillyl benzyl ether, which inhibits somatic embryogenesis of Japanese larch (Larix leptolepis Gordon). Plant Cell Physiol. 46:445–453; 2005a.

    Article  PubMed  CAS  Google Scholar 

  • Umehara, M.; Ogita, S.; Sasamoto, H.; Eun, C.; Matsubayashi, Y.; Sakagami, Y.; Kamada, H. Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon). Plant Sci. 169:901–907; 2005b.

    Article  CAS  Google Scholar 

  • West, M. A. L.; Harada, J. J. Embryogenesis in higher plants: an overview. Plant Cell 5: 1361–1369; 1993.

    Article  PubMed  Google Scholar 

  • Wurtele, E. S.; Wang, H.; Durgerian, S.; Nikolau, B. J.; Ulrich, T. Characterization of a gene that is expressed early in somatic embryogenesis of Daucus carota. Plant Physiol. 102:303–312; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Yeung, E. C.; Meinke, D. W. Embryogenesis in angiosperms: development of the suspensor. Plant Cell 5:1371–1381; 1993.

    Article  PubMed  Google Scholar 

  • Zimmerman, J. L. Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5:1411–1423; 1993.

    Article  PubMed  Google Scholar 

Download references

Acknowledgment

This work was supported in part by a Grant-in-Aid from the Research for the Future Program of the Japan Society for the Promotion of Science (JSPS-RFTF00L01601).

Author information

Authors and Affiliations

  1. Gene Research Center, University of Tsukuba, Tsukuba, 305-8572, Japan

    Mikihisa Umehara & Hiroshi Kamada

  2. Biotechnology Research Center, Toyama Prefectural University, Kosugi, 939-0398, Japan

    Shinjiro Ogita

  3. Graduate School of Environmental and Information Sciences, Yokohama National University, Hodogaya, 240-8501, Japan

    Hamako Sasamoto

  4. Molecular Characterization Team, RIKEN, Wako, 351-0198, Japan

    Hiroyuki Koshino

  5. Biomolecular Characterization Team, RIKEN, Wako, 351-0198, Japan

    Takemichi Nakamura

  6. Plant Function Laboratory, RIKEN, Wako, 351-0198, Japan

    Tadao Asami & Shigeo Yoshida

  7. Department of Biotechnology, Fukuoka Agricultural Research Center, Chikushino, 818-8549, Japan

    Mikihisa Umehara

Authors
  1. Mikihisa Umehara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shinjiro Ogita
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hamako Sasamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroyuki Koshino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takemichi Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tadao Asami
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shigeo Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiroshi Kamada
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mikihisa Umehara.

Additional information

Editor: R. J. Newton

Rights and permissions

Reprints and permissions

About this article

Cite this article

Umehara, M., Ogita, S., Sasamoto, H. et al. Identification of a factor that complementarily inhibits somatic embryogenesis with vanillyl benzyl ether in Japanese larch. In Vitro Cell.Dev.Biol.-Plant 43, 203–208 (2007). https://doi.org/10.1007/s11627-006-9016-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-006-9016-3

Keywords

Search

Navigation