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The influence of culture media on embryonic renal collecting duct cell differentiation

  • Growth, Differentiation And Senescence
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Summary

During kidney development the embryonic ampullar collecting duct (CD) epithelium changes its function. The capability for nephron induction is lost and the epithelium develops into a heterogeneously composed epithelium consisting of principal and intercalated cells. Part of this development can be mimicked under in vitro conditions, when embryonic collecting duct epithelia are isolated from neonatal rabbit kidneys and kept under perfusion culture. The differentiation pattern is quite different when the embryonic collecting duct epithelia are cultured in standard Iscove’s modified Dulbecco’s medium as compared to medium supplemented with additional NaCl. Thus, the differentiation behavior of embryonic CD epithelia is unexpectedly sensitive.

To obtain more information about how much influence the medium has on cell differentiation, we tested medium 199, basal medium Eagle, Williams’ medium E, McCoys 5A medium, and Dulbecco’s modified Eagle medium under serum-free conditions. The experiments show that in general, all of the tested media are suitable for culturing embryonic collecting duct epithelia. According to morphological criteria, there is no difference in morphological epithelial cell preservation. The immunohistochemical data reveal two groups of expressed antigens. Constitutively expressed antigens such as cytokeratin 19, PCD 9, Na/K ATPase, and laminin are present in all cells of the epithelia independent of the culture media used. In contrast, a group of antigens detected by mab 703, mab 503, and PNA is found only in individual series. Thus, each culture medium produces epithelia with a very specific cell differentiation pattern.

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References

  1. Aigner, J.; Kloth, S.; Minuth, W. W. Transitional differentiation patterns of principal and intercalated cells during renal collecting duct development. Epithelial Cell Biol. 4:121–130; 1995.

    PubMed  CAS  Google Scholar 

  2. Dulbecco, R.; Freeman, G. Iscove’s modified Eagle medium. Virology 8:396; 1959.

    Article  PubMed  CAS  Google Scholar 

  3. Durbeej, M.; Ekblom, P. Dystroglycan and laminins: glycoconjugates involved in branching epithelial morphogenesis. Exp. Lung Res. 23:109–118; 1997.

    PubMed  CAS  Google Scholar 

  4. Eagle, H. Basal medium eagle. Proc. Soc. Exp. Biol. Med. 89:362; 1965.

    Google Scholar 

  5. Emmons, C.; Kurtz, I. Functional characterization of three intercalated cell subtypes in the rabbit outer cortical collecting duct. J. Clin. Invest. 93:417–423; 1994.

    Article  PubMed  CAS  Google Scholar 

  6. Evan, A. P.; Satlin, L. M.; Gattone, V. H.; Connors, B.; Schwartz, G. J. Postnatal maturation of rabbit renal collecting duct. II. Morphological observations. Am. J. Physiol. 261:F91–107; 1991.

    PubMed  CAS  Google Scholar 

  7. Gerdes, J.; Li, L.; Schlueter, C.; Duchrow, M.; Wohlenberg, C.; Gerlach, C. Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am. J. Pathol. 138:867–873; 1991.

    PubMed  CAS  Google Scholar 

  8. Gross, P.; Minuth, W. W.; Kriz, W.; Frömter, E. Electric properties of renal collecting duct principal cell epithelium. Pflügers Arch. 406:380–386; 1986.

    Article  PubMed  CAS  Google Scholar 

  9. Hayashi, M.; Iyori, M.; Yamaji, Y.; Saruta, G. Effects of in vivo and in vitro alkali treatment of intracellular pH regulation of OMCD cells. Am. J. Physiol. 265:F729–735; 1993.

    PubMed  CAS  Google Scholar 

  10. Isabelle, M. E.; Githens, S.; Moses R. L., Bartell, C. K. Culture of rat renal medullary tissue in media made hyperosmotic with NaCl and urea. J. Exp. Zool. 269:308–318; 1994.

    Article  PubMed  CAS  Google Scholar 

  11. Kim, J.; Tisher, C. C.; Madsen, K. M. Differentiation of intercalated cells in developing rat kidney: an immunohistochemical study. Am. J. Physiol. 266:F977–990; 1994.

    PubMed  CAS  Google Scholar 

  12. Kleinmann, J. G.; Tipnis, P.; Pscheidt, P. H(+)-K(+)-ATPase of rat inner medullary collecting duct in primary culture. Am. J. Physiol. 265:F698–704; 1993.

    Google Scholar 

  13. Kloth, S.; Aigner, J.; Brandt, E.; Moll, R.; Minuth, W. W. Histochemical markers reveal an unexpected heterogenous composition of the renal embryonic collecting duct epithelium. Kidney Int. 44:527–536; 1993.

    PubMed  CAS  Google Scholar 

  14. Kloth, S.; Gmeiner, T.; Aigner, J.; Jennings, M. L.; Röckl, W.; Minuth, W. W. Transitional stages in the development of the rabbit renal collecting duct. Differentiation 63:21–32; 1998.

    Article  PubMed  CAS  Google Scholar 

  15. McCoy, T. A.; Maxwell, M.; Kruse, P. F. McCoys 5A medium. Proc. Exp. Biol. Med. 100:115; 1959.

    CAS  Google Scholar 

  16. Minuth, W. W.; Gross, P.; Gilbert, P.; Kashgarian, M. Expression of the α-subunit of Na/K-ATPase in renal collecting duct epithelium during development. Kidney Int. 31:1104–1112; 1987.

    PubMed  CAS  Google Scholar 

  17. Minuth, W. W. Neonatal rabbit kidney cortex in culture as tool for the study of collecting duct formation and nephron differentiation. Differentiation 36:12–22; 1987.

    Article  PubMed  CAS  Google Scholar 

  18. Minuth, W. W.; Dermietzel, R.; Kloth, S.; Hennerkes, B. A new method culturing renal cells under permanent superfusion and producing a luminal-basal medium gradient. Kidney Int. 41:215–219; 1992.

    PubMed  CAS  Google Scholar 

  19. Minuth, W. W.; Fietzek, W.; Kloth, S.; Stöckl, G.; Aigner, J.; Röckl, W.; Kubitza, M.; Dermietzel, R. Aldosterone modulates the development of PNA binding cell isoforms within renal collecting duct epithelium. Kidney Int. 44:337–344; 1993.

    Google Scholar 

  20. Minuth, W. W.; Aigner, J.; Kloth, S.; Steiner, P.; Tauc, M.; Jennings, M. L. Culture of embryonic renal collecting duct epithelium in a gradient container. Pediatr. Nephrol. 11:140–147; 1997.

    Article  PubMed  CAS  Google Scholar 

  21. Minuth, W. W.; Aigner, J.; Kubat, B.; Kloth, S. Improved differentiation of renal tubular epithelium in vitro: potential for tissue engineering. Exp. Nephrol. 5:10–17; 1997.

    PubMed  CAS  Google Scholar 

  22. Minuth, W. W.; Steiner, P.; Strehl, R.; Kloth, S.; Tauc, M. Electrolyte environment modulates differentiation in embryonic renal collecting duct epithelia. Exp. Nephrol. 5:414–422; 1997.

    PubMed  CAS  Google Scholar 

  23. Minuth, W. W.; Steiner, P.; Strehl, R.; Schumacher, K.; de Vries, U.; Kloth, S. Modulation of cell differentiation in perfusion culture. Exp. Neprhol.: in press.

  24. Moll, R.; Franke, W.; Schiller, D. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24; 1982.

    Article  PubMed  CAS  Google Scholar 

  25. Morgan, I.; Morton, P.; Parker, G. Medium 199. Proc. Soc. Exp. Biol. Med. 73:1; 1950.

    PubMed  CAS  Google Scholar 

  26. Obermüller, N.; Kränzlin, B.; Verma, R.; Gretz, N.; Kriz, W.; Witzgall, R. Renal osmotic stress-induced cotransporter: expression in the newborn, adult and postischemic rat kidney. Kidney Int. 52:1584–1592; 1997.

    Article  PubMed  Google Scholar 

  27. Ohtaka, A.; Muto, S.; Nemoto, J.; Kawakami, K.; Nagano, K.; Asano, Y. Hyperosmolality stimulates Na-K-ATPase gene expression in inner medullary collecting duct cells. Am. J. Physiol. 270:F728–738; 1996.

    PubMed  CAS  Google Scholar 

  28. Sariola, H.; Sainio, K. The tip-top branching ureter. Curr. Opin. Cell Biol. 9:877–884; 1997.

    Article  PubMed  CAS  Google Scholar 

  29. Satlin, L. M.; Matsumoto, T.; Schwartz, G. J. Postnatal maturation of rabbit renal collecting duct. III. Peanut lectin binding intercalated cells. Am. J. Physiol. 262:F199–208; 1992.

    PubMed  CAS  Google Scholar 

  30. Saxén, L. Organogenesis of the kidney. In: Barlow, P. W.; Green, P. B.; Wylie, C. C., ed. Developmental and cell biology series. Vol. 19. Cambridge: Cambridge University Press; 1987:13.

    Google Scholar 

  31. Sorokin, L.; Ekblom, P. Development of tubular and glomerular cells of the kidney. Kidney Int. 41:657–664; 1992.

    PubMed  CAS  Google Scholar 

  32. Steiner, P.; Strehl, R.; Kloth, S.; Tauc, M.; Minuth, W. W. In vitro development and preservation of specific features of collecting duct epithelial cells from embryonic rabbit kidney are regulated by the electrolyte environment. Differentiation 62:193–202; 1997.

    Article  PubMed  CAS  Google Scholar 

  33. Strehl, R.; Kloth, S.; Aigner, J.; Steiner, P.; Minuth, W. W. PCD Amp1, a new antigen at the interface of the embryonic collecting duct epithelium and the nephrogenic mesenchyme. Kidney Int. 52:1469–1477; 1997.

    Article  PubMed  CAS  Google Scholar 

  34. Tauc, M.; Koechlin, N.; Jamous, M.; Ouaghi, E. M.; Gastineau, M.; Le Moal, C.; Poujeol, P. Principal and intercalated cells in primary cultures of rabbit renal collecting tubules revealed by monoclonal antibodies. Biol. Cell 76:55–65; 1992.

    Article  PubMed  CAS  Google Scholar 

  35. Vilar, J.; Gilber, T.; Moreau, E.; Merlet-Bénichou, C. Metanephros organogenesis is highly stimulated by vitamin A derivatives in organ culture. Kidney Int. 49:1478–1487; 1996.

    PubMed  CAS  Google Scholar 

  36. Weller, A.; Sorokin, L.; Illgen, E. M.; Ekblom, P. Development and growth of mouse embryonic kidney in organ culture and modulation of development by soluble growth factors. Dev. Biol. 144:248–261; 1991.

    Article  PubMed  CAS  Google Scholar 

  37. Williams, G. M.; Gunn, J. M. Long-term cell culture of adult rat liver epithelial cells. Exp. Cell Res. 89:139; 1974.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Cellular and Molecular Physiology, University of Nice, Nice, France

    Michel Tauc

  2. Department of Anatomy, University of Regensburg, Universitätsstrasse 31, D-93053, Regensburg, Germany

    Karl Schumacher, Raimund Strehl, Sabine Kloth & Will W. Minuth

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  1. Karl Schumacher
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  2. Raimund Strehl
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  3. Sabine Kloth
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  4. Michel Tauc
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  5. Will W. Minuth
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Schumacher, K., Strehl, R., Kloth, S. et al. The influence of culture media on embryonic renal collecting duct cell differentiation. In Vitro Cell.Dev.Biol.-Animal 35, 465–471 (1999). https://doi.org/10.1007/s11626-999-0053-1

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  • DOI: https://doi.org/10.1007/s11626-999-0053-1

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