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Ligand-dependent and -independent interactions with the transforming growth factor type II and I receptor subunits reside in the aminoterminal portion of the ectodomain of the type III subunit

  • Growth, Differentiation And Senescence
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Summary

The type III receptor for transforming growth factor beta (TGFβ), which exhibits no kinase activity, binds TGFβ1 and TGFβ2 and is involved in assembly and activity of the multi-subunit TGFβ signal transduction complex. Recently we showed that TGFβ receptor type III (TβRIII) can participate in a complex composed of the dimeric TGFβ ligand and a type III, II, and I receptor subunit. The interaction of the TβRIII subunit with TβRII is TGFβ-dependent, whereas interaction with TβRI is TGFβ-independent. Here we use coexpression of the three types of TGFβ receptors in baculoviral-infected insect cells to determine which parts of the unglycosylated TβRIII receptor participate in the binding of TGFβ, the TGFβ-dependent interaction with TβRII and the TGFβ-independent interaction with TβRI. The results suggest that the first 500 amino acid residues in the aminoterminal portion of TβRIII exhibit all three properties.

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Author notes

  1. Akiyoshi Taniguchi

    Present address: Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, 274, Chiba, Japan

  2. Koichi Matsuzaki

    Present address: Third Department of Internal Medicine, Kansai Medical University, 10-15 Fumizonocho, Moriguchi, 570, Osaka, Japan

Authors and Affiliations

  1. Albert B. Alkek Institute of Biosciences and Technology, Center for Cancer Biology and Nutrition, Department of Biochemistry & Biophysics, Texas A&M University, 2121 West Holcombe Boulevard, 77030-3303, Houston, Texas

    Akiyoshi Taniguchi, Koichi Matsuzaki, Katsuya Nakano, Mikio Kan & Wallace L. Mckeehan

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  1. Akiyoshi Taniguchi
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  2. Koichi Matsuzaki
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  3. Katsuya Nakano
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  4. Mikio Kan
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  5. Wallace L. Mckeehan
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Taniguchi, A., Matsuzaki, K., Nakano, K. et al. Ligand-dependent and -independent interactions with the transforming growth factor type II and I receptor subunits reside in the aminoterminal portion of the ectodomain of the type III subunit. In Vitro Cell.Dev.Biol.-Animal 34, 232–238 (1998). https://doi.org/10.1007/s11626-998-0129-3

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  • DOI: https://doi.org/10.1007/s11626-998-0129-3

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