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Application of surfactin for mycoplasma inactivation in virus stocks

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References

  1. Baumgart, F.; Kluge, B.; Ullrich, C., et al. Identification of amino acid substitutions in the lipopeptide surfactin using 2D NMR spectroscopy. Biochem. Biophys. Res. Commun. 177:998–1005; 1991.

    Article  PubMed  CAS  Google Scholar 

  2. Drexler, H. G.; Gignac, S. M.; Hu, Z. B., et al. Treatment of mycoplasma contamination in a large panel of cell cultures. In Vitro Cell. Dev. Biol. 30A:344–347; 1994.

    CAS  Google Scholar 

  3. Hosono, K.; Suzuki, H. Morphological transformation of Chinese hamster cells by acylpeptides, inhibitors of cAMP phosphodiesterases, produced by Bacillus subtilis. J. Biol. Chem. 260:11252–11255; 1985.

    PubMed  CAS  Google Scholar 

  4. Hu, M.; Buck, C.; Jacobs, D., et al. Application of PCR for detection and identification of mycoplasma contamination in virus stocks. In Vitro Cell. Dev. Biol. 31A:710–716; 1995.

    Google Scholar 

  5. Ikoev, V. N.; Gonski, G. M.; Dzagurov, S. G. A method of ultracentrifugation used for control of live viral vaccines with regard to removal of mycoplasma. Vopr. Virusol. 18:625–628; 1973.

    PubMed  CAS  Google Scholar 

  6. Linn, M. L.; Bellett, A. J. D.; Parsons, P. G., et al. Complete removal of mycoplasma from viral preparations using solvent extraction. J. Virol. Methods 52:51–54; 1995.

    Article  PubMed  CAS  Google Scholar 

  7. Nissen, E.; Vollenbroich, D.; Pauli, G. Comparison of PCR preparation methods for mycoplasma detection in cell cultures. In Vitro Cell. Dev. Biol. 32A:463–464; 1996.

    Google Scholar 

  8. Reed, L. J.; Muench, H. A simple method for estimating fifty percent endpoints. Am. J. Hyg. 27:493–497; 1938.

    Google Scholar 

  9. Schmidt, J.; Erfle, V. Elimination of mycoplasmas from cell cultures and establishment of mycoplasma-free cell lines. Exp. Cell Res. 152:565–570; 1984.

    Article  PubMed  CAS  Google Scholar 

  10. Uphoff, C. C.; Gignac, S. M.; Drexler, H. G. Mycoplasma contamination in human leukemia cell lines. II. Elimination with various antibiotics. J. Immunol. Methods 149:55–62; 1992.

    PubMed  CAS  Google Scholar 

  11. van Kuppeveld, F. J. M.; Johannsson, K.-E.; Galama, J. M. D., et al. Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl. Environ. Microbiol. 60:149–152; 1994.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Virology, Robert Koch Institute, Nordufer 20, 13353, Berlin, Germany

    Eberhard Nissen & Georg Pauli

  2. Max-Volmer-Institut für Biophysikalische Chemie und Biochemie Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, 10587, Berlin, Germany

    Joachim Vater & Dirk Vollenbroich

Authors
  1. Eberhard Nissen
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  2. Georg Pauli
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  3. Joachim Vater
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  4. Dirk Vollenbroich
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Nissen, E., Pauli, G., Vater, J. et al. Application of surfactin for mycoplasma inactivation in virus stocks. In Vitro Cell.Dev.Biol.-Animal 33, 414–415 (1997). https://doi.org/10.1007/s11626-997-0056-8

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  • DOI: https://doi.org/10.1007/s11626-997-0056-8

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