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Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice

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Abstract

Premature ovarian failure (POF) is a refractory disease; one of the most important goals of treatment is to improve fertility. In the study, collagen scaffold loaded with human umbilical cord–derived mesenchymal stem cells (collagen/UC-MSCs) transplantation in POF mice preserved ovarian function, as supported by increased estrogen (E2) and anti-Mullerian hormone (AMH) levels, increased ovarian volume, and an increased number of antral follicles. Immunohistochemistry results of Ki67 indicated transplantation of collagen/UC-MSCs promoted granulosa cell proliferation, which is crucial to oocyte maturation and follicular development. Additionally, transplantation of collagen/UC-MSCs significantly promoted ovarian angiogenesis with the increased expression of CD31. In general, collagen/UC-MSCs transplantation probably is an effective therapeutic strategy of POF.

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Funding

Haixiang Sun is supported by National Nature Science Foundation of China (31571189), Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01030501), Jiangsu Province Social Development Project (BE2018602). This work is also supported from Lijun Ding by grants National Key Research and Development Program of China (2018YFC1004700), National Nature Science Foundation of China (81871128, 81571391, 30900847), Nanjing Medical Science Development Project (JQX14004, ZKX16042), and Jiangsu Provincial Medical Youth Talent (QNRC2016006). Shanshan Wang is supported by National Nature Science Foundation of China (81601277).

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Authors and Affiliations

  1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing, 210029, Jiangsu, China

    Yanjun Yang & Haixiang Sun

  2. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, Jiangsu, China

    Yanjun Yang, Lei Lei, Shanshan Wang, Xiaoqiang Sheng, Guijun Yan, Lu Xu, Jingyu Liu, Mengyuan Liu, Xin Zhen, Lijun Ding & Haixiang Sun

  3. Department of Obstetrics and Gynecology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, Jiangsu, China

    Lei Lei

  4. Clinical Center for Stem Cell Research, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China

    Lijun Ding

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  1. Yanjun Yang
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  2. Lei Lei
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  3. Shanshan Wang
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  4. Xiaoqiang Sheng
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  6. Lu Xu
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  8. Mengyuan Liu
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  9. Xin Zhen
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  10. Lijun Ding
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  11. Haixiang Sun
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Correspondence to Lijun Ding or Haixiang Sun.

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The authors declare that they have no conflict of interest.

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Editor: Tetsuji Okamoto

Yanjun Yang, Lei Lei, and Shanshan Wang are equal contributors

Electronic supplementary material

Supplementary Fig. 1

The body weight of POF mice after collagen/UC-MSCs transplantation. (a) The body weight of mice underwent different treatments. (PNG 29 kb)

High resolution image (TIF 144 kb)

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Yang, Y., Lei, L., Wang, S. et al. Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice. In Vitro Cell.Dev.Biol.-Animal 55, 302–311 (2019). https://doi.org/10.1007/s11626-019-00337-4

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  • DOI: https://doi.org/10.1007/s11626-019-00337-4

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