Abstract
Type 2 diabetes (T2D) is a condition with insufficient insulin production or in the setting of insulin resistance with many origins including intestinal microbiota-related molecular mechanism. Insulin-degrading enzyme (IDE) is responsible for insulin breakdown in various tissues and is known as a potential drug target for T2D. Here, we assessed the effects of cell-free supernatant (CFS) and UV-killed Lactobacillus casei IBRC_M10711 on IDE expression, IDE activity, and insulin degradation in Caco-2 cell line. It was found that CFS and UV-killed L. casei IBRC_M10711 led to lower expression of IDE. UV-killed L. casei IBRC_M10711 significantly inhibited IDE activity but CFS did not. Insulin degradation was affected with none of them. In conclusion, L. casei IBRC_M10711 is effective on IDE expression and its activity, but not on insulin degradation. Future studies are recommended to explore the effect of this probiotic on other substrates of IDE.
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This research study is funded by the Tehran University of Medical Sciences, International Campus (TUMS-IC). The authors thank the staffs of Pharmacology and Toxicology laboratory, Faculty of Pharmacy, for their collaborations.
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Neyazi, N., Mohammadi Farsani, T., Nouri, Z. et al. Potential efficacy of Lactobacillus casei IBRC_M10711 on expression and activity of insulin degrading enzyme but not insulin degradation. In Vitro Cell.Dev.Biol.-Animal 53, 12–19 (2017). https://doi.org/10.1007/s11626-016-0083-4
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DOI: https://doi.org/10.1007/s11626-016-0083-4