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RyR channel-mediated increase of cytosolic free calcium level signals cyclin B1 degradation during abortive spontaneous egg activation in rat

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Abstract

In few mammalian species including rat, post-ovulatory aging induces abortive spontaneous egg activation (SEA), which is morphologically characterized by exit from metaphase-II (M-II) arrest. A possibility exists that the RyR channel-mediated insufficient increase of cytosolic free Ca2+ level could be one of the causes for post-ovulatory aging-induced abortive SEA. To test this possibility, eggs collected after 17 h post-hCG surge were cultured with or without various concentrations of nifedipine (NF), ruthenium red (RR), and KN-93 for 3 h in vitro. Morphological changes characteristic of abortive SEA, cytosolic free Ca2+ level, cyclin B1 level, and meiotic status were analyzed. Data of the present study indicate that NF and RR inhibited post-ovulatory aging-induced abortive SEA in a concentration-dependent manner. Further, RR protected against RyR channel as well as caffeine-mediated increase of cytosolic free Ca2+ level. In addition, KN-93 inhibited post-ovulatory aging-induced abortive SEA in a concentration-dependent manner. An increase of cytosolic free Ca2+ level was associated with a reduction of cyclin B1 level during post-ovulatory aging-induced abortive SEA. These data indirectly suggest the involvement of RyR channels in the increase of cytosolic free Ca2+ level. The increased cytosolic free Ca2+ level triggers cyclin B1 degradation possibly through CaMK-II activity during post-ovulatory aging-induced abortive SEA in rat eggs cultured in vitro.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Cell Physiology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India

    Karuppanan V. Premkumar & Shail K. Chaube

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  1. Karuppanan V. Premkumar
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  2. Shail K. Chaube
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Correspondence to Shail K. Chaube.

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Editor: T. Okamoto

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Premkumar, K.V., Chaube, S.K. RyR channel-mediated increase of cytosolic free calcium level signals cyclin B1 degradation during abortive spontaneous egg activation in rat. In Vitro Cell.Dev.Biol.-Animal 50, 640–647 (2014). https://doi.org/10.1007/s11626-014-9749-y

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  • DOI: https://doi.org/10.1007/s11626-014-9749-y

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