Abstract
Human melanocyte stem cells (MSCs) or melanoblasts are not well-investigated owing to the devoid of suitable culture system. Establishing cell lines of MSCs and/or their progenies from human hair follicles will provide a better opportunity to satisfy clinical needs and to enable a deeper understanding of hair-related diseases. In the present study, we cultured melanocytes derived from human fetal hair follicles, perform immunocytochemistry and Fontana Masson staining on them, and employed atomic force microscopy (AFM) and scanning electron microscopy to observe their subtle morphologies. The results show that the cultured melanocytes have a bipolar or tripolar appearance, which obviously differ from cultured epidermal melanocytes. Compared to cells derived from adult human hair follicles, these cells display a high proliferative capability and exhibit a clonal growth behavior. At the second passage, all these cells were positive for immunocytochemical staining with the NKI/beteb monoclonal antibody and Fontana Masson staining. Under AFM, the cells exhibited rounded, oval, triangular, or quadrangular perikarya, from which two or three dendrites arose. The dendritic arbor was not homogeneous but appeared as spindle-shaped dendritic swellings, knob-like processes, without any filopodia arising from the dendrites or the cell body. Without using a feeder layer, we successfully obtained the clonal growth of melanocytes from human fetal HFs, suggesting that the medium was suitable for the growth of MSCs and their progenies.
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Acknowledgments
The authors are very grateful to Professor V. J. Hearing, Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD, for English language element of this paper.
The work was supported by the National Natural Science Foundation of China (no. 81171516) and the Science and Technological Fund of Anhui Province for Outstanding Youth (no. 8040106819).
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Zhang, Rz., Zhu, Wy., Li, Hz. et al. Culture of amelanotic melanocytes derived from human fetal hair follicles. In Vitro Cell.Dev.Biol.-Animal 49, 689–694 (2013). https://doi.org/10.1007/s11626-013-9649-6
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DOI: https://doi.org/10.1007/s11626-013-9649-6