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Polyoxypregnane glycoside from Dregea volubilis extract inhibits IL-1β-induced expression of matrix metalloproteinase via activation of NF-κB in human chondrocytes

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Abstract

Interleukin-1β (IL-1β) induces the expression of matrix metalloproteinases (MMPs) implicated in cartilage and joint degradation in osteoarthritis (OA) and rheumatoid arthritis (RA). Polyoxypregnane glycoside (PPG), active compound was identified from Dregea volubilis extract by chemical analysis, shown to exert chondroprotective effects in cartilage explant models. However, no studies have been undertaken for the molecular investigation of whether PPG constituents protect the human articular chondrocyte (HAC). In the present studies, HAC was co-treated with IL-1β and PPG. The expression of MMPs, type II collagen, phosphorylation of mitogen-activated protein kinases (MAPKs) and NF-κB signaling pathway were determined by Western immunoblotting. PPG (6.25–25 μM) decreased the IL-1β-induced HA release from chondrocyte to culture medium. The mode of action of PPG was likely mediated through inhibiting expression of MMP-1, -3 and -13 in the medium, which was associated with the inhibition of mRNA expression. PPG had no effect on IL-1β-induced phosphorylation of MAPK pathway. Conversely, PPG decreased phosphorylation of IκB kinase and IκBα degradation. Taken together, these results indicate that PPG may inhibit cartilage degradation in OA and may also be used as nutritional supplement for maintaining joint integrity and function.

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Acknowledgment

The authors would like to acknowledge financial support from the Thailand Excellence Centre for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University and Chiang Mai Graduate School for the financial support.

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Authors and Affiliations

  1. Thailand Excellence Center for Tissue Engineering and Stem Cells, Chiang Mai University, Chiang Mai, 50200, Thailand

    Akanit Itthiarbha, Thanyaluck Phitak, Peraphan Pothacharoen & Prachya Kongtawelert

  2. Laboratory of Natural Products and Center for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Lampang Rajabhat University, Lampang, 52100, Thailand

    Saksri Sanyacharernkul & Wilart Pompimon

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  1. Akanit Itthiarbha
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  2. Thanyaluck Phitak
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  3. Saksri Sanyacharernkul
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  4. Peraphan Pothacharoen
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  6. Prachya Kongtawelert
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Correspondence to Prachya Kongtawelert.

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Editor: T. Okamoto

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Itthiarbha, A., Phitak, T., Sanyacharernkul, S. et al. Polyoxypregnane glycoside from Dregea volubilis extract inhibits IL-1β-induced expression of matrix metalloproteinase via activation of NF-κB in human chondrocytes. In Vitro Cell.Dev.Biol.-Animal 48, 43–53 (2012). https://doi.org/10.1007/s11626-011-9475-7

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