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Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines

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Abstract

We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).

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Acknowledgements

This work was funded by the NorthWest Regional Development Agency (NWDA); NWESCC is also supported by the Medical Research Council and the Manchester NIHR Biomedical Research Centre. The authors would like to thank Dr. Stuart Liddle, Head of Genetics at TCL, for his help in the interpretation of the genotypes produced for unequivocal identification of the derived lines.

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Author notes

  1. Maria V. Camarasa

    Present address: Development and Regeneration Program, Caubet-Cimera Fundation, Advanced Respiratory Medicine Centre, Joan March Hospital, Bunyola Rd km 12, 07110, Palma de Mallorca, Spain

Authors and Affiliations

  1. North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, Manchester, M13 3PT, UK

    Maria V. Camarasa, Robbie W. Kerr, Nicola Bates, Rachel A. Oldershaw, Melissa A. Baxter, Tristan R. Mckay, Daniel R. Brison & Susan J. Kimber

  2. North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation Trust, St Mary’s Hospital, Manchester, M13 OJH, UK

    Robbie W. Kerr, Sharon F. Sneddon, Lisa Shaw & Daniel R. Brison

  3. North West Regional Cytogenetics Unit, St Mary’s Hospital, Manchester Royal Infirmary, Central Manchester University Hospitals NHS Foundation Trust, Oxford Road, Manchester, M13 9WL, UK

    Fiona Small

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  1. Maria V. Camarasa
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Correspondence to Maria V. Camarasa or Susan J. Kimber.

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Editor: J. Denry Sato

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Camarasa, M.V., Kerr, R.W., Sneddon, S.F. et al. Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines. In Vitro Cell.Dev.Biol.-Animal 46, 386–394 (2010). https://doi.org/10.1007/s11626-010-9291-5

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  • DOI: https://doi.org/10.1007/s11626-010-9291-5

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