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Differentiation of human adipose-derived stem cells induced by recombinantly expressed fibroblast growth factor 10 in vitro and in vivo

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Abstract

The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo co-culture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology.

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Acknowledgments

This work was funded by National Natural Science Foundation of China (No. 30428001) and the Major Program of The Eleventh Five-Year Plan of PLA Medicine (No. 06G62). I would like to express my sincere gratitude to Dr. Liu for providing guidance, support, and expertise throughout the course of this work. I would also like to thank Min-juan Wu, Ph.D for her helpful suggestions and the critical evaluation of my work.

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Authors and Affiliations

  1. Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, Shanghai, 200062, China

    Xunyi Zhang

  2. Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, China

    Xunyi Zhang, Minjuan Wu & Houqi Liu

  3. Laboratory Diagnosis Department, Second Military Medical University affiliated Changhai Hospital, Shanghai, 200433, China

    Weiwei Zhang

  4. Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, Shanghai, 200062, China

    Jiangfan Shen

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  1. Xunyi Zhang
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  2. Minjuan Wu
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  3. Weiwei Zhang
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  4. Jiangfan Shen
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  5. Houqi Liu
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Correspondence to Xunyi Zhang.

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Editor: J. Denry Sato

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Zhang, X., Wu, M., Zhang, W. et al. Differentiation of human adipose-derived stem cells induced by recombinantly expressed fibroblast growth factor 10 in vitro and in vivo. In Vitro Cell.Dev.Biol.-Animal 46, 60–71 (2010). https://doi.org/10.1007/s11626-009-9240-3

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