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Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood

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Abstract

Primary fibroblast-like cells isolated from the peripheral blood of a healthy pig were immortalized by transduction of cells with a replication-defective retrovirus vector expressing the E6/E7 proteins of human papillomavirus type 16 (pLXSN-16E6E7). The immortalized cells grow rapidly in cell culture and exhibit a distinct cell surface phenotype that was positive for CD90, CD44, collagen I, and vimentin and negative for CD14 and MHC II. Additionally, these immortalized blood derived-fibroblast-like cells had the potential to differentiate into osteoblasts and adipocytes in vitro as evidenced by the deposition of calcium, increased alkaline phosphatase activity, upregulated osteogenic and adipogenic marker gene expression, and accumulation of fat droplets in cells when osteogenic (dexamethasone, ascorbic acid, and β-glycerophosphate) or adipogenic supplements (dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin) were added to the culture. Overall, the results suggest that the immortalized blood-derived fibroblast-like cells exhibit some of the features of mesenchymal precursor cells, which may have implications in tissue repair and remodeling process.

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Acknowledgements

We thank Dr. Hongmei Li for the technical help in oil red O staining. We also thank Dr. Jeff Clapper and Mr. Dean Peters for providing us with pig blood. This work was partly supported by the South Dakota Agriculture Experiment Station and by NSF/EPSCoR #EPS-0091948 and by the State of South Dakota.

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  1. Department of Biology and Microbiology, Center for Infectious Disease Research and Vaccinology, South Dakota State University, NPB 252D, Box 2140D, Brookings, SD, 57007, USA

    Xiuqing Wang & Daphne Moutsoglou

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  1. Xiuqing Wang
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  2. Daphne Moutsoglou
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Correspondence to Xiuqing Wang.

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Editor: J. Denry Sato

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Wang, X., Moutsoglou, D. Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood. In Vitro Cell.Dev.Biol.-Animal 45, 584–591 (2009). https://doi.org/10.1007/s11626-009-9231-4

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  • DOI: https://doi.org/10.1007/s11626-009-9231-4

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