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The effect of implantation on scaffoldless three-dimensional engineered bone constructs

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An Erratum to this article was published on 23 October 2009

Abstract

Our laboratory has previously developed scaffoldless engineered bone constructs (EBC). Bone marrow stromal cells (BMSC) were harvested from rat femur and cultured in medium that induced osteogenic differentiation. After reaching confluence, the monolayer of cells contracted around two constraint points forming a cylinder. EBCs were placed in small diameter (0.5905 × 0.0625 in.) or large diameter (0.5905 × 0.125 in.) silicone tubing and implanted intramuscularly in the hind limb of a rat. Bone mineral content (BMC) of the EBC was analyzed before implantation and at 1 and 2 mo following implantation and compared to that of native femur bone at different stages of development. Negligible BMC was observed in E-20 femur or EBCs prior to implantation. One-month implantation in both small and large tubing increased BMC in the EBC. BMC of EBC from large tubing was greater than in 14 d rat neonatal femurs, but was 2% and 3% of BMC content in adult bone after 1 and 2 mo of implantation, respectively. Alizarine Red and osteopontin staining of the EBCs before and after implantation confirmed increased bone mineralization in the implanted EBCs. Implanted EBCs also had extensive vascularization. Our data suggest that BMSC can be successfully used for the generation of scaffoldless EBC, and this model can be potentially used for the generation of autologous bone transplants in humans.

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Acknowledgments

This research was supported by DARPA Biomolecular Motors Program, US Air Force, AFOSR award number FA9550-05-1-0015, and by NSF, Civil and Mechanical Systems grant no. CMS9988693.

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Authors and Affiliations

  1. Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200, USA

    Michael J. Smietana & Lisa M. Larkin

  2. Materials Science and Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200, USA

    Fatima N. Syed-Picard

  3. Molecular and Integrative Physiology, Biomedical Engineering, University of Michigan, Biomedical Science Research Building (BSRB), 109 Zina Pitcher Place, Room #2025, Ann Harbor, MI, 48109-2200, USA

    Tatiana Kostrominova & Lisa M. Larkin

  4. Mechanical Engineering, University of Michigan, 2250 GG Brown, 2350 Hayward, Ann Arbor, MI, 48109, USA

    Jinjin Ma & Ellen M. Arruda

  5. Program in Macromolecular Science and Engineering, University of Michigan, 2250 GG Brown, 2350 Hayward, Ann Arbor, MI, 48109, USA

    Ellen M. Arruda

  6. Department of Anatomy and Cell Biology, Indiana University School of Medicine-Northwest, Gary, IN, 46409-1008, USA

    Tatiana Kostrominova

Authors
  1. Michael J. Smietana
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  2. Fatima N. Syed-Picard
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  3. Jinjin Ma
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  4. Tatiana Kostrominova
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  5. Ellen M. Arruda
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  6. Lisa M. Larkin
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Corresponding author

Correspondence to Lisa M. Larkin.

Additional information

Editor: J. Denry Sato

An erratum to this article can be found at http://dx.doi.org/10.1007/s11626-009-9251-0

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Smietana, M.J., Syed-Picard, F.N., Ma, J. et al. The effect of implantation on scaffoldless three-dimensional engineered bone constructs. In Vitro Cell.Dev.Biol.-Animal 45, 512–522 (2009). https://doi.org/10.1007/s11626-009-9216-3

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  • DOI: https://doi.org/10.1007/s11626-009-9216-3

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