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Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models

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Abstract

To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound 48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells (RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol 12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-α, IL-6, and IL-8 production from HMC-1 cells by 71.16% (P < 0.001), 86.72% (P < 0.001), and 44.6%, respectively. However, FKME had no cytotoxic effects on cell viability. In conclusion, FKME inhibited not only systemic anaphylaxis and ear swelling induced by compound 48/80 but also inhibited a PCA reaction induced by anti-DNP IgE in vivo. Treatment with FKME showed significant inhibitory effects on histamine, TNF-α, IL-6, and IL-8 release from mast cells.

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Acknowledgment

This study was supported by VestibuloCochlear Research Center of Wonkwang University (R13-2002-055-01003-0), and a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, South Korea (01-PJ2-PG4-J201PT01-0006).

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Authors and Affiliations

  1. Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, South Korea

    In-Young Choi, Phil-Dong Moon, Hyun-Na Koo, Hyun-Ja Jeong, Jae-Young Um & Hyung-Min Kim

  2. College of Pharmacy, VestibuloCochlear Research Center of Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea

    In-Young Choi, Noh-Yil Myung, Su-Jin Kim, Ji-Hyun Lee, Se-Hee Han, Rae-Kil Park & Seung-Heon Hong

  3. Graduate School of Alternative Medicine, Kyonggi University, Seoul, 120-702, South Korea

    Hyun-Jea Sung

  4. College of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea

    Se-Hee Han, Goo Moon & Seung-Heon Hong

  5. Department of Biology, College of Natural Sciences, Kongju National University, Kongju, 314-701, South Korea

    Sung-Yum Seo

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  1. In-Young Choi
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Correspondence to Hyung-Min Kim.

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Choi, IY., Moon, PD., Koo, HN. et al. Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models. In Vitro Cell.Dev.Biol.-Animal 43, 215–221 (2007). https://doi.org/10.1007/s11626-007-9040-6

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