Abstract
Two cell lines, i.e., BmE-SWU1 and BmE-SWU2, were established from silkworm embryonic tissues of the reversion phase through primary culture in Grace’s medium supplemented with 20% fetal bovine serum. The BmE-SWU1 cell line mainly included diploid spindle cells and round cells, which were large and had severe heteroploidy karyotypes. The population doubling time of the 30th passage of the cell line was 58.7 hr. BmE-SWU2 cells were oblong or round, and small. The population doubling time for the 30th passage of the cell line was 46.6 hr. Of BmE-SWU2 cells 89.9% were diploid (2n = 56). Both strains were attached to epithelial-like cell lines and were susceptible to Bombyx mori nucleopolyhedroviruse (BmNPV). Inter simple sequence repeat (ISSR) fingerprinting of silkworm embryonic cell line was obtained.
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Acknowledgments
This work is supported by the National Basic Research Program of China under Grant No. 2005cb121000 and the National Natural Science Foundation of China under Grant No. 30471312, respectively.
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Pan, MH., Xiao, SQ., Chen, M. et al. Establishment and characterization of two embryonic cell lines of Bombyx mori . In Vitro Cell.Dev.Biol.-Animal 43, 101–104 (2007). https://doi.org/10.1007/s11626-006-9009-x
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DOI: https://doi.org/10.1007/s11626-006-9009-x