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Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1)

  • 2009 SSAT Quick Shot Presentation
  • Published:
Journal of Gastrointestinal Surgery Aims and scope

Abstract

Background

Protein is absorbed predominantly as di/tripeptides via H+/peptide cotransporter-1 (PEPT1). We demonstrated previously diurnal variations in expression and function of duodenal and jejunal but not ileal PEPT1; neural regulation of this pattern is unexplored.

Hypothesis

Complete abdominal vagotomy abolishes diurnal variations in gene expression and transport function of PEPT1.

Methods

Twenty-four rats maintained in a 12-h light/dark room [6AM–6PM] underwent abdominal vagotomy; 24 other rats were controls. Four weeks later, mucosal levels of mRNA and protein were measured at 9AM, 3PM, 9PM, and 3AM (n = 6 each) by quantitative real-time PCR and Western blots, respectively; transporter-mediated uptake of dipeptide (Gly–Sar) was measured by the everted-sleeve technique.

Results

Diurnal variation in mRNA, as in controls, was retained post-vagotomy in duodenum and jejunum (peak at 3PM, p < 0.05) but not in ileum. Diurnal variations in expression of protein and Gly–Sar uptake, however, were absent post-vagotomy (p > 0.3). Similar to controls, maximal uptake was in jejunum after vagotomy (V max, nmol/cm/min: jejunum vs. duodenum and ileum; 163 vs. 88 and 71 at 3AM; p < 0.04); K m remained unchanged.

Conclusions

Vagal innervation appears to mediate in part diurnal variations in protein expression and transport function of PEPT1, but not diurnal variation in mRNA expression of PEPT1.

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Acknowledgments

We would like to thank the Mary Elizabeth Groff Surgical Medical Research and Education Charitable Trust for the generous funding in support of this work. Also, we thank Deborah Frank for her superb secretarial expertise.

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Authors and Affiliations

  1. Gastrointestinal Research Unit and Department of Surgery, Mayo Clinic (GU 10-01), 200 1st Street SW, Rochester, MN, 55905, USA

    Hisham G. Qandeel, Fernando Alonso, David J. Hernandez, Judith A. Duenes, Ye Zheng, Jeffrey S. Scow & Michael G. Sarr

Authors
  1. Hisham G. Qandeel
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  2. Fernando Alonso
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  3. David J. Hernandez
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  4. Judith A. Duenes
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  5. Ye Zheng
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  6. Jeffrey S. Scow
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  7. Michael G. Sarr
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Corresponding author

Correspondence to Michael G. Sarr.

Additional information

Presented in part at the Society for Surgery of the Alimentary Tract 50th Annual Meeting, May 30 to June 4, 2009, Chicago, Illinois and published in abstract form in Gastroenterology, May 2009.

Research supported in part by a grant from the Mary E. Groff Foundation and NIH R01 DK 39337-18 (MGS)

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Qandeel, H.G., Alonso, F., Hernandez, D.J. et al. Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1). J Gastrointest Surg 13, 1976–1985 (2009). https://doi.org/10.1007/s11605-009-0984-6

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  • DOI: https://doi.org/10.1007/s11605-009-0984-6

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