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S100A4 mRNA is a Diagnostic and Prognostic Marker in Pancreatic Carcinoma

  • Original Article
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Journal of Gastrointestinal Surgery Aims and scope

Abstract

Objective

The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer.

Materials and Methods

We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction.

Results

IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028).

Conclusion

S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.

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Authors and Affiliations

  1. Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Fukuoka, 812-8582, Japan

    Naoki Ikenaga, Kenoki Ohuchida, Kazuhiro Mizumoto, Jun Yu, Hayato Fujita, Kohei Nakata, Junji Ueda, Norihiro Sato, Eishi Nagai & Masao Tanaka

  2. Department of Advanced Medical Initiatives, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

    Kenoki Ohuchida

  3. Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

    Norihiro Sato

  4. Kyushu University Hospital Cancer Center, Fukuoka, Japan

    Kazuhiro Mizumoto

Authors
  1. Naoki Ikenaga
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  2. Kenoki Ohuchida
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  3. Kazuhiro Mizumoto
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  4. Jun Yu
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Corresponding author

Correspondence to Kenoki Ohuchida.

Additional information

Supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and grants from the Takeda Science Foundation, Pancreas Research Foundation of Japan, and Nakajima Foundation.

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Ikenaga, N., Ohuchida, K., Mizumoto, K. et al. S100A4 mRNA is a Diagnostic and Prognostic Marker in Pancreatic Carcinoma. J Gastrointest Surg 13, 1852–1858 (2009). https://doi.org/10.1007/s11605-009-0978-4

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  • DOI: https://doi.org/10.1007/s11605-009-0978-4

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