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miR-206 inhibits renal cell cancer growth by targeting GAK

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Abstract

Renal cell cancer (RCC) remains one of the most lethal types of cancer in adults. MicroRNAs (miRNAs) play key roles in the pathogenesis of RCC. The role of miR-206 in RCC has not been fully understood. The purpose of this study was to examine the role of miR-206 in the regulation of proliferation and metastasis of RCC and the possible mechanism. miR-206 expression was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in RCC cell lines (786-O and OS-RC-2 cells) and clinical samples. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] method, colony formation and transwell assay were used to detect the tumor-suppressing ability of miR-206 in RCC. Luciferase assay was performed to verify the precise target of miR-206. The results showed that the expression of miR-206 was significantly down-regulated in RCC tissues and cells. The expression level of cyclin G-associated kinase (GAK), a master regulator of tumor proliferation and metastasis, was up-regulated with the decrease in miR-206 in RCC tissues as well as RCC cell lines. In addition, the miR-206 inhibitor promoted the proliferation, migration and invasion of 786-O and OS-RC-2 cells. Bioinformatics combined with luciferase and Western blot assays revealed that miR-206 inhibited the expression of GAK. Moreover, miR-206 regulates RCC cell growth partly through targeting GAK. Our study indicated that miR-206 functions as a tumor suppressor in regulating the proliferation, migration and invasion of RCC by directly targeting GAK, and it holds promises as a potential therapeutic target for RCC.

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Authors and Affiliations

  1. Department of Urology, Huazhong University of Science and Technology, Wuhan, 430030, China

    Chao Wei  (卫 超), Shen Wang  (王 珅), Zhang-qun Ye  (叶章群) & Zhi-qiang Chen  (陈志强)

  2. Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Chao Wei  (卫 超), Shen Wang  (王 珅), Zhang-qun Ye  (叶章群) & Zhi-qiang Chen  (陈志强)

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  1. Chao Wei  (卫 超)
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  2. Shen Wang  (王 珅)
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  3. Zhang-qun Ye  (叶章群)
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  4. Zhi-qiang Chen  (陈志强)
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Correspondence to Zhi-qiang Chen  (陈志强).

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Wei, C., Wang, S., Ye, Zq. et al. miR-206 inhibits renal cell cancer growth by targeting GAK. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 852–858 (2016). https://doi.org/10.1007/s11596-016-1674-8

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  • DOI: https://doi.org/10.1007/s11596-016-1674-8

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