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Effects and clinical significance of pentoxifylline on the oxidative stress of rats with diabetic nephropathy

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Summary

Diabetic nephropathy (DN) is a common and serious clinical complication of diabetes and presently there are no effective ways to prevent its occurrence and progression. Recent studies show that pentoxifylline (PTX) can improve renal hemodynamics, reduce urinary protein excretion, and alleviate or delay renal failure in DN patients. In this study, we focused on the anti-oxidative stress effect of PTX on alleviating renal damages of DN using rat models. DN rats were established with injection of streptozotocin. Blood glucose, urinary protein excretion, serum cystatin C, renal biopsy, superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and renal homogenate and renal nitrotyrosine levels were analyzed before and 12 weeks after the treatment of PTX. Before treatment, all the DN rats had elevated blood glucose, increased urinary protein excretion and elevated serum cystatin C. Morphologically, DN rats exhibited renal tissue damages, including swelling and fusions of foot processes of podocytes under electron microscope. Masson staining revealed blue collagen deposition in glomeruli and renal interstitium. With treatment of PTX, symptoms and renal pathological changes of DN rats were alleviated. Furthermore, the MDA levels were increased and the SOD levels were decreased in the serum and kidneys of DN rats, and these changes were reversed by PTX. The expression of nitrotyrosine was up-regulated in DN rat model and down-regulated by PTX, indicating that PTX was able to inhibit oxidative reactions in DN rats. PTX could alleviate renal damage in DN, which may be attributable to its anti-oxidative stress activity.

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Authors and Affiliations

  1. Department of Endocrinology, Shanghai Second People’s Hospital, Shanghai, 200011, China

    Zeng-mei An  (安增梅), Xing-gang Dong  (董兴刚) & Yuan Guo  (过源)

  2. Laboratory Animal Science Center, Fudan University, Shanghai, 200032, China

    Jia-liang Zhou  (周佳亮) & Tao Qin  (秦涛)

Authors
  1. Zeng-mei An  (安增梅)
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  2. Xing-gang Dong  (董兴刚)
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  3. Yuan Guo  (过源)
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  4. Jia-liang Zhou  (周佳亮)
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  5. Tao Qin  (秦涛)
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Corresponding author

Correspondence to Zeng-mei An  (安增梅).

Additional information

This project was supported by grants from the Research Program of Huangpu District Science and Technology Committee of Shanghai, China (No. 2012-HGG-5), and the Program for Outstanding Academic Leaders of Health System of Huangpu District of Shanghai, China (No. 2013-18).

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An, Zm., Dong, Xg., Guo, Y. et al. Effects and clinical significance of pentoxifylline on the oxidative stress of rats with diabetic nephropathy. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 35, 356–361 (2015). https://doi.org/10.1007/s11596-015-1437-y

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  • DOI: https://doi.org/10.1007/s11596-015-1437-y

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