Summary
Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic (37–38°C) ischemia, mild hypothermic (31–32°C) ischemia, hyperthermic (41–42°C) ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20-min ischemia. The expression of c-fos protein and the levels of malondialdehyde (MDA) and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-fos protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion following 20-min ischemia as compared with the sham-operated group (P<0.01). The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group (P<0.01). It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.
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References
Campos F, Blanco M, Barral D, et al. Influence of temperature on ischemic brain: basic and clinical principles. Neurochem Int, 2012, 60(5):495–505
Yenari MA, Han HS. Neuroprotective mechanisms of hypothermia in brain ischaemia. Nat Rev Neurosci, 2012, 13(4):267–278
Zhang H, Zhou M, Zhang J, et al. Therapeutic effect of post-ischemic hypothermia duration on cerebral ischemic injury. Neurol Res, 2008, 30(4):332–336
Zhang H, Zhou M, Zhang J, et al. Initiation time of post-ischemic hypothermia on the therapeutic effect in cerebral ischemic injury. Neurol Res, 2009, 31(4):336–339
Zhang H, Xu G, Zhang J, et al. Mild hypothermia reduces ischemic neuron death via altering the expression of p53 and bcl-2. Neurol Res, 2010, 32(4):384–389
Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke, 1979, 10(3):267–272
Prakasa Babu P, Yoshida Y, Su M, et al. Immunohistochemical expression of Bcl-2, Bax and cytochrome c following focal cerebral iscbemia and effect of hypothermia in rat. Neurosci Lett, 2000, 291(3):196–200
Eberspächer E, Werner C, Engelhard K, et al. Long-term effects of hypothermia on neuronal cell death and the concentration of apoptotic proteins after incomplete cerebral ischemia and reperfusion in rats. Acta Anaesthesiol Scand, 2005, 49(4):477–487
Jieyong B, Zhong W, Shiming Z, et al. Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats. Neurosurg Rev, 2006, 29(2):168–172
Eberspächer E, Werner C, Engelhard K, et al. The effect of hypothermia on the expression of the apoptosis-regulating protein Bax after incomplete cerebral ischemia and reperfusion in rats. J Neurosurg Anesthesiol, 2003, 15(3):200–208
Satoh K, Niwa M, Binh NH, et al. Increase of galectin-3 expression in microglia by hyperthermia in delayed neuronal death of hippocampal CA1 following transient forebrain ischemia. Neurosci Lett, 2011, 504(3):199–203
Tsai YW, Yang YR, Wang PS, et al. Intermittent hypoxia after transient focal ischemia induces hippocampal neurogenesis and c-Fos expression and reverses spatial memory deficits in rats. PLoS One, 2011, 6(8):e24001
Akaji K, Suga S, Fujino T, et al. Effect of intra-ischemic hypothermia on the expression of c-Fos and c-Jun, and DNA binding activity of AP-1 after focal cerebral ischemia in rat brain. Brain Res, 2003, 975(1–2):149–157
Mancuso A, Derugin N, Hara K, et al. Mild hypothermia decreases the incidence of transient ADC reduction detected with diffusion MRI and expression of c-fos and hsp70 mRNA during acute focal ischemia in rats. Brain Res, 2000, 887(1):34–45
Kobayashi MS, Asai S, Ishikawa K, et al. Global profiling of influence of intra-ischemic brain temperature on gene expression in rat brain. Brain Res Rev, 2008, 58(1): 171–191
Abas F, Alkan T, Goren B, et al. Neuroprotective effects of postconditioning on lipid peroxidation and apoptosis after focal cerebral ischemia/reperfusion injury in rats. Turk Neurosurg, 2010, 20(1):1–8
Woo CW, Lee BS, Kim ST, et al. Correlation between lactate and neuronal cell damage in the rat brain after focal ischemia: An in vivo 1H magnetic resonance spectroscopic (1H-MRS) study. Acta Radiol, 2010, 51(3): 344–350
Natale JA, D’Alecy LG. Protection from cerebral ischemia by brain cooling without reduced lactate accumulation in dogs. Stroke, 1989, 20(6):770–777
Karaszewski B, Wardlaw JM, Marshall I, et al. Early brain temperature elevation and anaerobic metabolism in human acute ischaemic stroke. Brain, 2009, 132(Pt 4):955–964
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This project was supported by the Key Projects of Scientific Research Funds from Health Department of Hubei Province (No. JX5A04), China.
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Zhang, H., Li, L., Xu, Gy. et al. Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 34, 354–358 (2014). https://doi.org/10.1007/s11596-014-1282-4
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DOI: https://doi.org/10.1007/s11596-014-1282-4