Summary
In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
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References
Park KK, Liu K, Hu Y, et al. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science, 2008,322(5903):963–966
Christie KJ, Webber CA, Martinez JA, et al. PTEN inhibition to facilitate intrinsic regenerative outgrowth of adult peripheral axons. J Neurosci, 2010,30(27):9306–9315
Walker CL, Walker MJ, Liu NK, et al. Systemic bisperoxovanadium activates Akt/mTOR, reduces autophagy, and enhances recovery following cervical spinal cord injury. PLoS One, 2012,7(1):e30012
Yang P. Promoting the repair of CNS by targeting PTEN. Prog Phys Sci (Chinese), 2010,41(4):313–316s
Abe N, Borson SH, Gambello MJ, et al. Mammalian target of rapamycin (mTOR) activation increases axonal growth capacity of injured peripheral nerves. J Biol Chem, 2010,285(36):28 034–28 043
Liu K, Lu Y, Lee JK, et al. PTEN deletion enhances the regenerative ability of adult corticospinal neurons. Nat Neurosci, 2010,13(9):1075–1081
Kurimoto T, Yin Y, Omura K, et al. Long-distance axon regeneration in the mature optic nerve: contributions of oncomodulin, cAMP, and pten gene deletion. J Neurosci, 2010,30(46):15654–15663
Zhu Y, Hoell P, Ahlemeyer B, et al. Implication of PTEN in production of reactive oxygen species and neuronal death in in vitro models of stroke and Parkinson’s disease. Neurochem Int, 2007,50(3):507–516
Wu HR, Wang YC, Cai QY. Roles of PTEN in neuronal injury and brain disorders. Yixue Zongshu (Chinese). 2010,16(11):1607–1610
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This study was supported by Science and Technology Planning Project of Guangdong Province, China (No. 2011B031800205).
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Lin, Q., Chen, Hy., Li, Hs. et al. Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 34, 76–80 (2014). https://doi.org/10.1007/s11596-014-1234-z
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DOI: https://doi.org/10.1007/s11596-014-1234-z