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Pingyangmycin-regulated expressions of adhesion molecules in human venous malformation endothelial cells

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Summary

Pingyangmycin (bleomycin A5 hydrochloride, PYM) is one of the anti-neoplastic agents which have been commonly used to treat venous malformations. However, the underlying mechanism by which PYM treats venous malformations remains poorly understood. It was reported that venous endothelial cells could recruit neutrophils via adhesion molecules (E-selectin, ICAM-1, ICAM-3, VCAM-1) during the acute/chronic inflammation and subsequent histological fibrosis after sclerotherapy with PYM. This study explored if the expression of E-selectin, ICAM-1, ICAM-3 and VCAM-1 in human venous malformation endothelial cells could be affected by PYM. HVMECs were cultured from human venous malformation tissue. Expressions of E-selectin, ICAM-1, ICAM-3 and VCAM-1 on HVMECs in response to PYM were analyzed by cell ELISA. The relative levels of mRNA expression in the cells were semi-quantified. The results showed that PYM up-regulated the expressions of E-selectin, ICAM-3, VCAM-1 and ICAM-1 in both time- and concentration-dependent manner. Our findings suggested that PYM could induce the expression of adhesion molecules in HVMECs, which might be a possible mechanism by which sclerotherapy by intralesional injection of PYM treats venous malformations.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yulin Jia  (贾玉林)

  2. Department of Oral and Maxillofacial Surgery, College & Hospital of Stomatology, Wuhan University, Wuhan, 430070, China

    Jun Jia  (贾 俊) & Yifang Zhao  (赵怡芳)

Authors
  1. Yulin Jia  (贾玉林)
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  2. Jun Jia  (贾 俊)
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  3. Yifang Zhao  (赵怡芳)
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Corresponding author

Correspondence to Yifang Zhao  (赵怡芳).

Additional information

This project was supported by the Natural Science Foundation of Hubei province, China (No. 2010CDB07907).

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Jia, Y., Jia, J. & Zhao, Y. Pingyangmycin-regulated expressions of adhesion molecules in human venous malformation endothelial cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 760–766 (2012). https://doi.org/10.1007/s11596-012-1031-5

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  • DOI: https://doi.org/10.1007/s11596-012-1031-5

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