Summary
This study examined in vitro effect of lipoxin A4 (LXA4) on interleukin-1β (IL-1β) production of monocytes and its possible mechanism in severe preeclampsia (PE). Peripheral venous blood was drawn from 15 patients with severe preeclampsia (PE group) and 20 normal pregnant women (control group) to prepare monocytes which were then treated with LXA4 at different concentrations of 0, 10, 100 nmol/L respectively. IL-1β level in the supernatant of monocytes was detected by enzyme linked immunoassay. The [Ca2+]i of monocytes was measured by laser scanning confocal microscopy. The results showed that the IL-1β level and the [Ca2+]i of monocytes in the PE group were significantly higher than those in the control group. LXA4 significantly decreased the generation of IL-1β in a dose-dependent manner in the PE group. After treatment with 100-nmol/L LXA4, in the PE group, the [Ca2+]i concentration of monocytes was significantly reduced. It was concluded that LXA4 may inhibit the IL-1β production of monocytes from severe preeclampsia women by inhibiting extracellular calcium influx.
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References
Redman CW, Sacks GP, Sargent IL. Preeclampsia: An excessive maternal inflammatory response to pregnancy. Am J Obstet Gynecol, 1999,180(2):499–506
Saito S, Shiozaki A, Nakashima A, et al. The role of the immune system in preeclampsia. Mol Aspects Med, 2007,28(2):192–209
Redman CW, Sargent IL. Pre-eclampsia, the placenta and the maternal systemic inflammatory response-a review. Placenta, 2003,24(4):21–27
Kocyigit Y, Atamer Y, Atamer A, et al. Changes in serum levels of leptin, cytokines and lipoprotein in pre-eclamptic and normotensive pregnant women. Gynecol Endocrinol, 2004,19(5):267–273
Guven MA, Coskun A, Ertas IE, et al. Association of maternal serum CRP, IL-6, TNF-alpha, homocysteine, folic acid and vitamine B 12 levels with the severity of preeclampsia and fetal birth weight. Hypertension Pregnancy, 2009,28(2):190–200
EL-baradle SM, Mahmoud M, Makhlouf HH. Elevated serum levels of interleukin-15, interleukin-16, and human chorionic gonadotropin in women with preeclampsia. J Obstet Gynaecol Can, 2009,31(2):142–148
Vural P, Degirmencioglu S, Saral NY, et al. Tumor necrosis factor alpha, interleukin-6 and interleukin-10 polymorphisms in preeclampsia. J Obstet Gynaecol Res, 2010,36(1):64–71
Beckmann I, Efraim SB, Vervoort M, et al. Tumor necrosis factor-alpha in whole blood cultures of preeclamptic patients and healthy pregnant and nonpregnant women. Hypertens Pregnancy, 2004,23(3): 319
Starosta V, Pazdrak K, Boldogh I, et al. Lipoxin A4 counterregulates GM-CSF signaling in eosinophilic granulocytes. J Immunol, 2008,181(12):8688–8699
Bonnans C, Vachier I, Chavis C, et al. Lipoxins are potential endogenous antiinflammatory mediators in asthma. Am J Respir Crit Care Med, 2002,165(11): 1531–1535
Merched AJ, Ko K, Gotlinger KH, et al. Atherosclerosis: Evidence for impairment of resolution of vascular inflammation governed by specific lipid mediators. FASEB J, 2008,22(10):3595–3606
Carvalho RR, Pellizzon CH, Justulin L, et al. Effect of mangiferin on the development of periodontal disease: involvement of lipoxin A4, anti-chemotaxic action in leukocyte rolling. Chem Biol Interact, 2009,179(2–3): 344–350
Fukunaga K. Cyclooxygenase 2 derived lipid mediators play a pivotal role in resolution of acute lung injury. Nihon Kokyuki Gakkai Zasshi, 2008,46(2):156–164
Yacoubian S, Serhan CN. New endogenous anti-inflammatory and proresolving lipid mediators: implications for rheumatic diseases. Nat Clin Pract Rheumatol, 2007,3(10):570–579
Machado FS, Esper L, Dias A, et al. Native and aspirin-triggered lipoxins control innate immunity by inducing proteasomal degradation of TRAF6. J Exp Med, 2008,205(5):1077–1086
Mangino MJ, Brounts L, Harms B, et al. Lipoxin biosynthesis in inflammatory bowel disease. Prostaglandins Other Lipid Mediat, 2006,79(1–2):84–92
von Dadelszen P, Wilkins T, Redman CW. Maternal peripheral blood leukocytes in normal and pre-eclamptic pregnancies. Br J Obstet Gynaecol, 1999,106(6):576–581
ACOG Committee on Practice Bulletins-Obstetrics. ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Obstet Gyneco, 2002,99: 159–167
Holthe MR, Staff AC, Berge LN, et al. Leukocyte adhesion molecules and reactive oxygen species in preeclampsia. Obstet Gynecol, 2004,103(5 Pt 1):913–922
Luppi P, Deloia JA. Monocytes of preeclamptic women spontaneously synthesize pro-inflammatory cytokines. Clin Immunol, 2006,118(2–3):268–275
Chen M, Divangahi M, Gan H, et al. Lipid mediators in innate immunity against tuberculosis: opposing roles of PGE2 and LXA4 in the induction of macrophage death. J Exp Med, 2008,205(12):2791–2801.
Pinton P, Giorgi C, Siviero R, et al. Calcium and apoptosis: ER-mitochondria Ca2+ transfer in the control of apoptosis. Oncogene, 2008,27(50):6407–6418
Feske S, Giltnane J, Dolmetsch R, et al. Gene regulation mediated by calcium signals in T lymphocytes. Nat Immunol, 2001,2(4):316–324
Wilson L, Butcher CJ, Kellie S. Calcium ionophore A23187 induces interleukin-8 gene expression and protein secretion in human monocytic cells. FEBS Lett, 1993,325(3):295–298
Brough D, Le Feuvre RA, Wheeler RD, et al. Ca2+ stores and Ca2+ entry differentially contribute to the release of IL-1 beta and IL-1 alpha from murine macrophages. J Immunol, 2003,170(6):3029–3036
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Wang, J., Huang, Y., Huang, Y. et al. Effect of lipoxin A4 on IL-1β production of monocytes and its possible mechanism in severe preeclampsia. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 767–770 (2010). https://doi.org/10.1007/s11596-010-0655-6
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DOI: https://doi.org/10.1007/s11596-010-0655-6