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Biocompatibility of KLD-12 peptide hydrogel as a scaffold in tissue engineering of intervertebral discs in rabbits

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Summary

KLD-12 peptide with a sequence of AcN-KLDLKLDLKLDL-CNH2 was synthesized and its biocompatibility was assessed in animals. Rabbit MSCs were cultured in the hydrogel for 2 weeks. Live cells were counted by using Calcein-AM/PI fluorescence staining. MTT was employed to assess the viability of MSCs cultured in KLD-12 peptide solution of 0.01%, 0.03%, and 0.05%. Hemolysis test, skin irritation test and implantation test were conducted to evaluate its biocompatibility with host tissues. Our results demonstrated that the MSCs in hydrogel grew well and maintained round shape. Cell survival rate was 92.15% (mean: 92.15%±1.17%) at the 7th day and there was no difference in survival rate between day 7 and day 14. Cell proliferation test showed that the A value of the KLD-12 solutions was not significantly different from that of control groups (complete culture media) (P>0.05) at the 24th and 48th h. The hemolysis rate of KLD-12 solution was 0.112%. Skin irritation test showed that the skin injected with KLD-12 solution remained normal and the score of skin irritation was 0. The histological examination with HE staining exhibited that the skin layers were clear and there was no infiltration with neutrophilic granulocytes and lymphocytes. It is concluded that KLD-12 peptide hydrogel had a good biocompatibility with host rabbit and MSCs, and KLD-12 peptide hydrogel can provide an appropriate microenvironment for MSCs.

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Authors and Affiliations

  1. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jianhua Sun  (孙建华), Qixin Zheng  (郑启新), Yongchao Wu  (吴永超), Yudong Liu  (刘郁东), Xiaodong Guo  (郭晓东) & Weigang Wu  (伍卫刚)

  2. Department of Orthopedics, the First Affiliated Hospital of Shihezi University, Xinjiang, 832008, China

    Jianhua Sun  (孙建华)

Authors
  1. Jianhua Sun  (孙建华)
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  2. Qixin Zheng  (郑启新)
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  3. Yongchao Wu  (吴永超)
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  4. Yudong Liu  (刘郁东)
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  5. Xiaodong Guo  (郭晓东)
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  6. Weigang Wu  (伍卫刚)
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Corresponding author

Correspondence to Qixin Zheng  (郑启新).

Additional information

This project was supported by a grant from the National High Technology Research and Development Program of China (Program 863) (No. 2006AA02A124).

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Sun, J., Zheng, Q., Wu, Y. et al. Biocompatibility of KLD-12 peptide hydrogel as a scaffold in tissue engineering of intervertebral discs in rabbits. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 173–177 (2010). https://doi.org/10.1007/s11596-010-0208-z

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  • DOI: https://doi.org/10.1007/s11596-010-0208-z

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