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Heme oxygenase-1 expression in rats with acute lung rejection and implication

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Summary

This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat→Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group. The severity of acute rejection was graded on the basis of the morphologic changes of the lung samples stained with HE. The expression of HO-1 protein in lung tissue was detected by using immunohistochemistry and Western blot, and HO-1 mRNA activity was assayed by RT-PCR. The results showed that the expression of HO-1 protein was significantly increased with the acute rejection grading in rats (P<0.01). As compared with control and ZnPP-treated groups, the severity of acute rejection was not alleviated and the grade not reduced significantly in CoPP-treated group (P>0.05). It was concluded that HO-1 protein might be involved in the pathological process of post-graft acute rejection. The expression of HO-1 protein was increased gradually with aggravation of acute rejection, and HO-1 protein might be used as an index to monitor acute rejection after lung transplantation.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Ke Jiang  (江 科), Jiangjun Wang  (王建军), Jinsong Li  (李劲松) & Jun Nie  (聂 君)

  2. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Lin Cheng  (程 琳)

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  1. Ke Jiang  (江 科)
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  2. Lin Cheng  (程 琳)
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  3. Jiangjun Wang  (王建军)
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  4. Jinsong Li  (李劲松)
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  5. Jun Nie  (聂 君)
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Jiang, K., Cheng, L., Wang, J. et al. Heme oxygenase-1 expression in rats with acute lung rejection and implication. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 84–87 (2009). https://doi.org/10.1007/s11596-009-0118-0

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  • DOI: https://doi.org/10.1007/s11596-009-0118-0

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