Summary
The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with pIRES2-EGFP-S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with pIRES2-EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with pIRES2-EGFP-K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs.
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Quansheng WANG and Ali ZHANG contributed equally to the manuscript.
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30600810).
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Wang, Q., Zhang, A., Li, R. et al. High glucose promotes the CTGF expression in human mesangial cells via serum and glucocorticoid-induced kinase 1 pathway. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 508–512 (2008). https://doi.org/10.1007/s11596-008-0504-z
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DOI: https://doi.org/10.1007/s11596-008-0504-z