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Inhibition of invasion and up-regulation of E-cadherin expression in human malignant melanoma cell line A375 by (-)-epigallocatechin-3-gallate

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Summary

The inhibitory effects of (-)-epigallocatechin-3-gallate (EGCG) on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pretreated with 20 μg/mL EGCG for 24, 48 and 72 h respectively and the E-cadherin expression was detected by Western blot analysis. A375 cells were also pretreated with different concentrations of EGCG (1, 5, 10 and 20 μg/mL) for 72 h and the expression of E-cadherin was measured by RT-PCR. The adhesion and invasion of A375 cells were tested by cell-matrigel adhesion assay and matrigel invasion assay respectively. The results showed that EGCG could significantly up-regulate the expression of E-cadherin time-and concentration-dependently (both P<0.05). Statistical analysis showed that A375 cells invasion was inhibited by EGCG and correlated with the up-regulation of E-cadherin expression. It was suggested that EGCG strongly inhibited invasion of A375 cells, and the inhibition mechanism was possibly associated with the up-regulation of E-cadherin expression.

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Authors and Affiliations

  1. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yan Wu  (吴 艳), Yun Lin  (林 云), Houjun Liu  (刘厚君) & Jiawen Li  (李家文)

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  1. Yan Wu  (吴 艳)
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  2. Yun Lin  (林 云)
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  3. Houjun Liu  (刘厚君)
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  4. Jiawen Li  (李家文)
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Wu, Y., Lin, Y., Liu, H. et al. Inhibition of invasion and up-regulation of E-cadherin expression in human malignant melanoma cell line A375 by (-)-epigallocatechin-3-gallate. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 356–359 (2008). https://doi.org/10.1007/s11596-008-0330-3

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  • DOI: https://doi.org/10.1007/s11596-008-0330-3

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