Summary
The neural stem cells in Wistar rats were cultured in vitro, purified, and transplanted into C6 glioma model in order to observe their biological characters and provide a basic foundation for treatment of neurological diseases by neural stem cell transplantation. The cells at hippocampal area from gestation 15-day rats were cultured in vitro, and frozen and preserved in liquid nitrogen. C6 tumor-bearing models (n=25) and neural stem cells transplantation models (n=35) were established. When the tumor grew to 3 to 4 weeks, 5 rats in each group were randomly selected for MRI examination. At different intervals, the rats were perfused and sampled for HE staining, GFAP and BrdU immunohistochemical staining. The results showed that after resuscitation of neural stem cells at 1–4 passages, the cell viability was 40%–63% with the difference being not significant. The cells could proliferate, passage, and most cells transplanted into glioma model survived. The mean survival time in neural stem cell transplantation group and control was 4.28 and 3.88 weeks respectively, and the average tumor size in the former was smaller than in the latter. It was concluded that embryonic neural stem cells in rats could proliferate and differentiate, and after resuscitation the biological characteristic and viability of the cells were not influenced. Neural stem cells had inhibitory effects on the growth of glioma cells and could prolong the survival of rat model.
Similar content being viewed by others
References
Aboody K S, Brown A, Rainov N G et al. Neural stem cells display extensive tropism for pathology in adult brain: Evidence from intracranial gliomas. PNAS, 2000,97(23):12 846–12 851
Ehtesham M, Kabos P, Gutierrez M A et al. Induction of glioblastoma apoptosis using neural stem cell-mediated delivery of tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res, 2002, 62(24): 7170–7174
Trojan J, Johnson T R, Rudin S D et al. Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA. Science, 1993, 259(5091):94–97
Honeth G, Staflin K, Kalliomaki S et al. Chemokine-directed migration of tumor-inhibitory neural progenitor cells towards an intracranially growing glioma. Exp Cell Res, 2006,312(8):1265–1276
Glass R, Synowitz M, Kronenberg G et al. Glioblastoma-induced attraction of endogenous neural precursor cells is associated with improved survival. J Neurosci, 2005,25(10):2637–2646
Song J H, Bellail A, Tse M C et al. Human astrocytes are resistant to Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis. J Neurosci, 2006,26(12):3299–3308
Ehtesham M, Black K L, Yu J S. Recent progress in immunotherapy for malignant glioma: treatment strategies and results from clinical trials. Cancer Control, 2004,11(3):192–207
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luo, J., Zhang, L., Tu, H. et al. Experimental study on treatment of glioma by embyonic neural stem cell transplnation in rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 571–575 (2007). https://doi.org/10.1007/s11596-007-0524-0
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11596-007-0524-0