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Effects of meloxicam on vascular endothelial growth factor and angiopoietin-2 expression in colon carcinoma cell line HT-29

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Summary

To investigate the effect of meloxicam, a selected NSAIDs, on cell growth, expression of VEGF and angiopointin-2 (Ang-2) protein in HT-29 cell line, cultured HT-29 cells were treated with meloxicam of various concentrations for various lengths of time. The proliferation of HT-29 was detected by cell counting kit-8 (CCK-8), the cell cycle was determined by flow cytometer and the levels of VEGF and Ang-2 protein in supernatants were examined by enzyme linked immunosorbent assay (ELISA). The mRNA expressions of VEGF and Ang-2 in cultured HT-29 were determined by real-time quantitative reverse-transcription polymerase chain reaction. Our results showed that treatment of meloxicam of different concentrations and for various lengths of time had a cytotoxicic effect on the cell proliferation of HT-29 cells in a concentration-dependant and time-dependant manner. Cell cycle analysis showed that the cells were mainly blocked in G0/G1 phase. The VEGF and Ang-2 protein levels in supernatants of the culture medium were decreased gradually in a concentration-dependent or time-dependent fashion. The mRNA expression of cox-2, VEGF and Ang-2 showed a gradual and concentration-dependent reduction. It is concluded that meloxicam can reduce the expression of VEGF and Ang-2 at the protein and mRNA level in colon carcinoma cell line.

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Authors and Affiliations

  1. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhang Ning  (张宁), Tao Kaixiong  (陶凯雄) & Huang Tao  (黄韬)

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  1. Zhang Ning  (张宁)
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  2. Tao Kaixiong  (陶凯雄)
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  3. Huang Tao  (黄韬)
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ZHANG Ning, female, born in 1979, Doctorial Candidate

This project was supported by a grant from R&D program of Hubei Provincial government (No. 2005AA304B09).

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Zhang, N., Tao, K. & Huang, T. Effects of meloxicam on vascular endothelial growth factor and angiopoietin-2 expression in colon carcinoma cell line HT-29. J. Huazhong Univ. Sc. Technol. 27, 399–402 (2007). https://doi.org/10.1007/s11596-007-0412-7

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  • DOI: https://doi.org/10.1007/s11596-007-0412-7

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