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Apoptosis and expression of protein TRAIL in granulosa cells of rats with polycystic ovarian syndrome

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Summary

The relationship between apoptosis of granulosa cells and follicle development arrest in polycystic ovarian syndrome (PCOS) rats, and the contribution of tumor necrosis factor related apoptosis inducing ligand (TRAIL) in apoptosis of granulosa cells were explored. By using sodium prasterone sulfate rat PCOS model was induced. The apoptosis of granulosa cells in ovaries of rats was observed by TdT-mediated dUTP-biotin nick end-labeling (TUNEL), and the expression of TRAIL protein and mRNA in granulosa cells was detected by using immunhistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) respectively. The apoptotic rate and the expression of protein TRAIL in granulosa cells were significantly higher in antral follicles from the PCOS rats than in those from the control rats (P<0.01, P<0.05). There was no significant difference in apoptotic rate and the expression of TRAIL protein in granulosa cells of preantral follicles between the PCOS rats and the control rats (P>0.05). No apoptosis and the expression of TRAIL protein in granulosa cells of primordial follicles were found in the two groups. The expression of TRAIL mRNA was significantly stronger in granulosa cells from the PCOS rats than in those from the control rats (P<0.01). It was suggested that the apoptotic rate in granulosa cells was significantly higher in antral follicle from the PCOS rats than in those from the control rats. TRAIL played a role in regulating the apoptosis of granulosa cells in PCOS rats.

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Authors and Affiliations

  1. Reproductive Medical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhang Juan  (张娟), Zhu Guijin  (朱桂金), Wang Xinrong  (王昕荣), Xu Bei  (徐蓓) & Hu Linli  (胡琳莉)

  2. Department of Obstetrics and Gynecology, PLA NO 161 Hospital, Wuhan, 430010, China

    Zhang Juan  (张娟)

Authors
  1. Zhang Juan  (张娟)
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  2. Zhu Guijin  (朱桂金)
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  3. Wang Xinrong  (王昕荣)
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  4. Xu Bei  (徐蓓)
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  5. Hu Linli  (胡琳莉)
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Corresponding author

Correspondence to Zhu Guijin  (朱桂金).

Additional information

ZHANG Juan, female, born in 1971, Doctor in Charge, M.D., Ph.D.

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Zhang, J., Zhu, G., Wang, X. et al. Apoptosis and expression of protein TRAIL in granulosa cells of rats with polycystic ovarian syndrome. J. Huazhong Univ. Sc. Technol. 27, 311–314 (2007). https://doi.org/10.1007/s11596-007-0324-6

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  • DOI: https://doi.org/10.1007/s11596-007-0324-6

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