Summary
This study examined the implication of EMT induced by TGF-β1 in pancreatic cancer invasion. TGF-β1 expression was determined in 29 cases of human pancreatic carcinoma (PC) by immunohistochemistry and the results were compared with those of pathological examination. Moreover, the effects of TGF-β1 on the phenotype and invasion of pancreatic cancer cell line Panc-1 were also investigated. TGF-β1 was detected in 12 cases (41.4 %) of PC. Significant correlation was found between the expression of TGF-β1 and lymph node involvement (P=0.047) and the depth of invasion (P=0.035). TGF-β1 obviously promoted EMT of Panc-1 cell lines and their invasion ability was substantially enhanced. TGF-β1 may promote the malignancy of pancreatic cancer by triggering EMT.
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References
Thiery J P. Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer, 2002,2:442–454
Bhowmick N A, Ghiassi M, Bakin A et al. Transforming growth factor-betal mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell, 2001,12:27–36
Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: a laboratory manual. 2rd ed. New York: Cold Spring Harbor Laboratory Press, 1989. 870–877
Ellenrieder V, Hendler S F, Ruhland C et al. TGF-beta-induced invasiveness of pancreatic cancer cells is mediated by matrix metalloproteinase-2 and the urokinase plasminogen activator system. Int J Cancer, 2001,93:204–211
Hazelbag S, Kenter G G, Gorter A et al. Prognostic relevance of TGF-beta1 and PAI-1 in cervical cancer. Int J Cancer, 2004,112:1020–1028
Xiong B, Gong L L, Zhang F et al. TGF beta1 expression and angiogenesis in colorectal cancer tissue. World J Gastroenterol, 2002,8:496–498
Janda E, Lehmann K, Killisch I et al. Ras and TGF[beta] cooperatively regulate epithelial cell plasticity and metastasis: dissection of Ras signaling pathways. J Cell Biol, 2002,156:299–313
Kasai H, Jeremy T A, Roger M M et al. TGF-β1 induces human alveolar epithelial to mesenchymal cell transition (EMT). Respir Res, 2005,6(1): 56
Sugimachi K, Tanaka S, Kameyama T et al. Transcriptional repressor snail and progression of human hepatocellular carcinoma. Clin Cancer Res, 2003,9(7):2657–2664
Gilles C, Polette M, Zahm J M et al. Vimentin contributes to human mammary epithelial cell migration. J Cell Sci, 1999,112:4615–4625
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This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30571817).
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Yin, T., Wang, C., Liu, T. et al. Implication of EMT induced by TGF-β1 in pancreatic cancer. J. Huazhong Univ. Sc. Technol. 26, 700–702 (2006). https://doi.org/10.1007/s11596-006-0619-z
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DOI: https://doi.org/10.1007/s11596-006-0619-z