Skip to main content

Advertisement

SpringerLink
Log in

Influence of three remineralization materials on physicochemical structure of demineralized enamel

  • Biomaterials
  • Published:
Journal of Wuhan University of Technology-Mater. Sci. Ed. Aims and scope Submit manuscript

Abstract

This study aimed to evaluate the effect of three inorganic materials:casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), calcium sodium phosphosilicate (CSP) and sodium fluoride (NaF) on their remineralization potential. CPP-ACP, CSP and NaF were applicated in demineralized enamel for 4 h and then all enamel samples were immersed in remineralization solution. After 10 days, all samples were prepared for microhardness test, scanning electron microscopy (SEM) observation and the elemental level (%) analysis by energy dispersive X-ray spectroscopy (EDX). The surface microhardness (SMH) values of three experimental groups were significantly higher than that of control group (P<0.05). The SMH value of NaF group was significantly higher than other experimental groups (P<0.05). The SEM micrographs showed that three remineralization materials caused distinct morphological changes and remineralized deposits were different from each other. EDX elemental analysis showed that there were significant differences in the Ca (wt%) and Ca:P molar ratio of the enamel layer among control and experimental groups. The Ca (wt%) and Ca:P molar ratio in NaF group were significantly higher than those of other experimental groups. There were no significant differences in P (wt%) among control and experimental groups (P<0.05). Three remineralization materials used in our study had the potential of remineralizing the demineralized enamel. NaF is the best and most feasible choice to be used in remineralizing the demineralized enamel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Margolis HC, Moreno EC. Physicochemical Perspectives on the Cariostatic Mechanisms of Systemic and Topical Fluorides[J]. J. Dent. Res., 1990, 69 Spec No: 606–613, 634–636

    Google Scholar 

  2. Hicks J, Garcia-Godoy F, Flaitz C. Biological Factors in Dental Caries: Role of Remineralization and Fluoride in the Dynamic Process of Demineralization and Remineralization (Part 3)[J]. J Clin Pediatr Dent, 2004, 28(3):203–214

    Google Scholar 

  3. Cochrane NJ, Cai F, Huq NL, et al. New Approaches to Enhanced Remineralization of Tooth Enamel[J]. J. Dent. Res., 2010, 89(11):1 187–1 197

    Article  Google Scholar 

  4. Benson PE, Shah AA, Millett DT, et al. Fluorides, Orthodontics and Demineralization: A Systematic Review[J]. J Orthod, 2005, 32(2): 102–114

    Article  Google Scholar 

  5. Reynolds EC, Cain CJ, Webber FL, et al. Anticariogenicity of Calcium Phosphate Complexes of Tryptic Casein Phosphopeptides in the Rat[J]. J. Dent. Res., 1995, 74(6): 1 272–1 279

    Article  Google Scholar 

  6. Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and Casein Phosphopeptide-Amorphous Calcium Phosphate[J]. J. Dent. Res., 2008, 87(4): 344–348

    Article  Google Scholar 

  7. Cross KJ, Huq NL, Palamara JE, et al. Physicochemical Characterization of Casein Phosphopeptide-Amorphous Calcium Phosphate Nanocomplexes[J]. J. Biol. Chem., 2005, 280(15): 15 362–15 369

    Article  Google Scholar 

  8. Yamaguchi K, Miyazaki M, Takamizawa T, et al. Effect of CPP-ACP Paste On Mechanical Properties of Bovine Enamel as Determined by an Ultrasonic Device[J]. J. Dent., 2006, 34(3): 230–236

    Article  Google Scholar 

  9. Kokubo T, Kim HM, Kawashita M. Novel Bioactive Materials with Different Mechanical Properties[J]. Biomaterials, 2003, 24(13): 2 161–2 175

    Article  Google Scholar 

  10. Burwell AK, Litkowski LJ, Greenspan DC. Calcium Sodium Phosphosilicate (NovaMin): Remineralization Potential[J]. Adv. Dent. Res., 2009, 21(1): 35–39

    Article  Google Scholar 

  11. Tung MS, Eichmiller FC. Amorphous Calcium Phosphates for Tooth Mineralization[J]. Compend. Contin. Educ. Dent., 2004, 25(9 Suppl 1): 9–13

    Google Scholar 

  12. Du Min Q, Bian Z, Jiang H, et al. Clinical Evaluation of a Dentifrice Containing Calcium Sodium Phosphosilicate (Novamin) for the Treatment of Dentin Hypersensitivity[J]. Am. J. Dent., 2008, 21(4): 210–214

    Google Scholar 

  13. Featherstone JD. The Science and Practice of Caries Prevention[J]. Journal of American Dental Association, 2000, 131:887–899

    Article  Google Scholar 

  14. Weir MD, Chow LC, Xu HH. Remineralization of Demineralized Enamel Via Calcium Phosphate Nanocomposite[J]. J. Dent. Res., 2012, 91(10): 979–984

    Article  Google Scholar 

  15. Cochrane NJ, Zero DT, Reynolds EC. Remineralization Models[J]. Adv. Dent. Res., 2012, 24(2): 129–132

    Article  Google Scholar 

  16. Amaechi BT, Ramalingam K, Mensinkai PK, et al. In Situ Remineralization of Early Caries by a New High-Fluoride Dentifrice[J]. Gen. Dent., 2012, 60(4): e186–e192

    Google Scholar 

  17. Khoroushi M, Mazaheri H, Manoochehri A. Effect of CPP-ACP Application On Flexural Strength of Bleached Enamel and Dentin Complex[J]. Oper. Dent., 2011, 36(4): 372–379

    Article  Google Scholar 

  18. Reynolds EC. Anticariogenic Complexes of Amorphous Calcium Phosphate Stabilized by Casein Phosphopeptides: A Review[J]. Spec. Care. Dentist., 1998, 18(1):8–16

    Article  Google Scholar 

  19. Aimutis WR. Bioactive Properties of Milk Proteins with Particular Focus On Anticariogenesis[J]. J. Nutr., 2004, 134(4):989S–995S

    Google Scholar 

  20. Cochrane NJ, Saranathan S, Cai F, et al. Enamel Subsurface Lesion Remineralisation with Casein Phosphopeptide Stabilised Solutions of Calcium, Phosphate and Fluoride[J]. Caries Res., 2008, 42(2): 88–97

    Article  Google Scholar 

  21. Srinivasan N, Kavitha M, Loganathan SC. Comparison of the Remineralization Potential of CPP-ACP and CPP-ACP with 900 Ppm Fluoride On Eroded Human Enamel: An in Situ Study[J]. Arch. Oral Biol., 2010, 55(7): 541–544

    Article  Google Scholar 

  22. Cerruti MG, Greenspan D, Powers K. An Analytical Model for the Dissolution of Different Particle Size Samples of Bioglass in TRISbuffered Solution[J]. Biomaterials, 2005, 26(24): 4 903–4 911

    Article  Google Scholar 

  23. Andersson OH, Kangasniemi I. Calcium Phosphate Formation at the Surface of Bioactive Glass in Vitro[J]. J. Biomed. Mater. Res., 1991, 25(8): 1 019–1 030

    Article  Google Scholar 

  24. Amaechi BT, Ramalingam K, Mensinkai PK, et al. In Situ Remineralization of Early Caries by a New High-Fluoride Dentifrice[J]. Gen Dent, 2012, 60(4): e186–e192

    Google Scholar 

  25. Al-Mullahi AM, Toumba KJ. Effect of Slow-Release Fluoride Devices and Casein Phosphopeptide/Amorphous Calcium Phosphate Nanocomplexes On Enamel Remineralization in Vitro[J]. Caries Res., 2010, 44(4): 364–371

    Article  Google Scholar 

  26. Langhorst SE, O’Donnell JN, Skrtic D. In Vitro Remineralization of Enamel by Polymeric Amorphous Calcium Phosphate Composite: Quantitative Microradiographic Study[J]. Dent. Mater., 2009, 25(7): 884–891

    Article  Google Scholar 

  27. Zhang LL, Li JY, Zhou XD, et al. Chemical and Crystallographic Study of Remineralized Surface On Initial Carious Enamel Treated with Galla Chinensis[J]. Scanning, 2009, 31(6): 236–245

    Google Scholar 

  28. Hara AT, Karlinsey RL, Zero DT. Dentine Remineralization by Simulated Saliva Formulations with Different Ca and Pi Contents[J]. Caries Res., 2008, 42(1):51–56

    Article  Google Scholar 

  29. Lambrou D, Larsen MJ, Fejerskov O, Tachos B. The Effect of Fluoride in Saliva On Remineralization of Dental Enamel in Humans[J]. Caries Res., 1981, 15(5): 341–345

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The State Key Laboratory Breding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China

    Jinmei Lei  (雷晋梅), Jinxin Guo, Dongjie Fu, Yake Wang, Liqun Zhou & Cui Huang  (黄翠)

  2. Center of Stomatology, TongJi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xijin Du

Authors
  1. Jinmei Lei  (雷晋梅)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinxin Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dongjie Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yake Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xijin Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liqun Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Cui Huang  (黄翠)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cui Huang  (黄翠).

Additional information

Funded by the National Natural Science Foundation of China (Nos. 81070852 and 81171010)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lei, J., Guo, J., Fu, D. et al. Influence of three remineralization materials on physicochemical structure of demineralized enamel. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 29, 410–416 (2014). https://doi.org/10.1007/s11595-014-0931-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11595-014-0931-6

Key words

Search

Navigation