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Phylogenetic analysis of Engleromyces sinensis and identification of cytochalasin D from culture

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Abstract

Engleromyces sinensis (Xylariales, Xylariaceae) is an important medicinal fungus in China. In the present study, its taxonomic status was confirmed by muti-locus DNA (ITS-LSU-RPB2-TUB2) phylogenetic analysis for the first time. The main active components of the stromata and cultured mycelium of E. sinensis were analyzed by high-performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) methods. Cytochalasin D was identified in stromata and mycelium, and no neoengleromycin was detected. Due to the lack of molecular data related to E. goetzei, the interspecific relationships of E. goetzei and E. sinensis need to be investigated further.

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All data generated or analysed during this study are included in this published article and its supplementary information files.

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Acknowledgements

We sincerely thank two anonymous reviewers for their valuable comments and embellishment on the revision of this paper, which is of great significance for the manuscript of this study.

Funding

This study was supported by the National Natural Science Foundation of China (No. 31870629).

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Conceptualization: Hao Zhou, Qiu-Tong Wang and Cheng-Lin Hou; data curation: Hao Zhou, Qiu-Tong Wang and Xin Tong; formal analysis: Hao Zhou, and Xin Tong; funding acquisition: Cheng-Lin Hou; methodology: Hao Zhou, Qiu-Tong Wang, Xin Tong, and Cheng-Lin Hou; project administration: Cheng-Lin Hou; resources: Hao Zhou, Qiu-Tong Wang, Xin Tong, and Cheng-Lin Hou; supervision: Cheng-Lin Hou; writing-original draft: Hao Zhou, Qiu-Tong Wang, and Xin Tong; writing-review and editing: Hao Zhou, Qiu-Tong Wang, Xin Tong, and Cheng-Lin Hou. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Cheng-Lin Hou.

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Hao Zhou and Qiu-Tong Wang are contributed equally to this work.

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Zhou, H., Wang, QT., Tong, X. et al. Phylogenetic analysis of Engleromyces sinensis and identification of cytochalasin D from culture. Mycol Progress 20, 1343–1352 (2021). https://doi.org/10.1007/s11557-021-01739-z

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  • DOI: https://doi.org/10.1007/s11557-021-01739-z

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