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Taxonomy and pathogenicity of Leptographium species associated with Ips subelongatus infestations of Larix spp. in northern China, including two new species

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Abstract

The larch bark beetle (Ips subelongatus), which occurs in larch plantations over a vast area of eastern Asia, infects both dying and fallen trees. When its population reaches a high density, the beetle may also infect healthy trees, resulting in tree decline and, eventually, death. Leptographium spp., in both their sexual and asexual states, are mainly associated with conifer-infesting bark beetles; some species are important tree pathogens. The aims of this study were to identify the Leptographium spp. associated with I. subelongatus infestations of Larix spp. in northern China and to examine their pathogenicity towards the tree. Morphological studies and phylogenetic approaches based on multilocus DNA sequence data (ITS2- partial r28S, partial β-tubulin, and EF-1α gene regions) showed that three Leptographium species occur in association with I. subelongatus in the areas investigated: Leptographium taigense, which is recorded in China for the first time, and two new species, namely L. innermongolicum sp. nov. and L. zhangii sp. nov. Leptographium innermongolicum is closely related to L. taigense, whereas L. zhangii belongs to the Grosmannia piceaperda species complex. The pathogenicity of these Leptographium species towards mature Larix spp. was tested by stem inoculation in forests. All inoculations only resulted in small lesions on the inner bark; therefore, the three Leptographium species were not considered to be pathogenic.

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Acknowledgments

This study was supported by the Special Fund for Forest Scientific Research in the Public Welfare (201204501), the National Natural Science Foundation of China (project no.: 31070571), and the Special Fund for Public Welfare Institutes of the Central Government (CAFRIFEEP201102). This work was conducted under the auspices of the Chinese Academy of Forestry International Cooperation and Innovation Team. Quan Lu gratefully acknowledges a postdoctoral scholarship granted by the Wallonia-Brussels International Excellent Grants Program. Cony Decock gratefully acknowledges the financial support received from the Belgian State and Belgian Federal Science Policy through the BCCM™ research program.

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Authors and Affiliations

  1. Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, 1 East Xiaofu, Xiang Shan Road, Haidian District, Beijing, 100091, China

    Xue-Wei Liu, Hui-Min Wang, Quan Lu, Yong-Xia Li & Xing-Yao Zhang

  2. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China

    Xue-Wei Liu, Hui-Min Wang, Quan Lu, Yong-Xia Li & Xing-Yao Zhang

  3. Wuqing Forestry Bureau, 301700, Tianjin, China

    Xue-Wei Liu

  4. Earth and Life Institute, Microbiology, Mycothèque de l’Université catholique de Louvain (MUCL), Croix du Sud 2 bte L.7.05.06, 1348, Louvain-la-Neuve, Belgium

    Cony Decock

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  1. Xue-Wei Liu
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Correspondence to Quan Lu.

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Liu, XW., Wang, HM., Lu, Q. et al. Taxonomy and pathogenicity of Leptographium species associated with Ips subelongatus infestations of Larix spp. in northern China, including two new species. Mycol Progress 16, 1–13 (2017). https://doi.org/10.1007/s11557-016-1245-1

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