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Nimbya and Embellisia revisited, with nov. comb for Alternaria celosiae and A. perpunctulata

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Abstract

Previous phylogenetic analyses revealed that species within the genera Nimbya and Embellisia reside within a large monophyletic clade that also includes the genera Alternaria, Ulocladium, Undifilum, Sinomyces, and Crivellia with Stemphylium as the sister taxon. This study expands upon previous work by including many contemporary species of each genus and utilizes molecular and morphological characters to further examine relationships. Maximum parsimony and Bayesian analysis reveals that Nimbya is not a monophyletic genus but is split into two phylogenetically distant clades, which have different and distinct conidial morphologies. One of these clades resides completely within Alternaria. Phylogenetic analyses also reveals that Embellisia does not form a monophyletic genus but is split into four monophyletic lineages. Moreover, several species of Embellisia cluster individually with clades that are predominantly Alternaria, Ulocladium, or Stemphylium, yet these Embellisia spp. possess morphological characters that are diagnostically Embellisia. Thus, these data reveal that both Nimbya and Embellisia are polyphyletic as currently defined and taxonomic restructuring is necessary in order to resolve conflict between historical morphological and contemporary molecular-based phylogenies.

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References

  • Berbee ML, Pirseyedi M, Hubbard S (1999) Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 91:964–977

    Article  CAS  Google Scholar 

  • Chen WQ, Lin XF, Zhang TY (1997) A new species of Nimbya. Mycosystema 2:106–108

    Google Scholar 

  • Crivelli PG (1983) U¨ ber die heterogene Ascomycetengattung Pleospora Rabh.: Vorshlag fu¨r eine Aufteilung.— Diss. ETH. Nr. 7318. Zu¨rich: ADAG Administration & Druck AG. 213 p

  • David JC, Coles K, Fisher J, Moss ST (2000) A new species of Embellisia from soil with high levels of heavy metals. Mycoscience 41:533–537

    Article  Google Scholar 

  • de Hoog GS, Muller PJ (1973) A new species of Embellisia associated with skin disease of hyacinths. Neth J Plant Path 79:85–93

    Article  Google Scholar 

  • de Hoog GS, Seigle-Murandi F, Steinman R, Eriksson KE (1985) A new species of Embellisia from the North Sea. J Microbio 51:409–413

    Google Scholar 

  • Eriksson OE, Hawksworth DL (1991) Notes on ascomycete systematics. Systema Ascomycetum 12:1–38

    Google Scholar 

  • Fuckel L (1863) Fungi rhenani exsiccate, Fasc. I, No. 1–100.

  • Goldman N, Anderson JP, Rodrigo AG (2000) Likelihood-based tests of topologies in phylogenetics. Syst Biol 49(4):652–670

    Article  PubMed  CAS  Google Scholar 

  • Hasegawa M, Kishino H (1989) Confidence limits on the maximum-likelihood estimate of the hominoid tree from mitochondrial-DNA sequences. Evolution 43:672–677

    Article  Google Scholar 

  • Hoes JA, Bruehl GW, Shaw CG (1965) A new Pseudostemphylium. Mycologia 57:904–912

    Article  Google Scholar 

  • Holcomb GE, Antonopoulos AA (1976) Alternaria alternantherae: A new species found on alligatorweed. Mycologia 68:1125–1129

    Google Scholar 

  • Hong SG, Cramer RA, Lawrence CB, Pryor BM (2005) Alt a1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet Biol 42:119–129

    Article  PubMed  CAS  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  PubMed  CAS  Google Scholar 

  • Johnson DA, Simmons EG, Miller JS, Stewart EL (2002) Taxonomy and pathology of Macrospora/Nimbya on some North American bulrushes (Scirpus spp.). Mycotaxon 84:413–428

    Google Scholar 

  • Maddison DR, Maddison WP (2003) MacClade 4.06: analysis of phylogeny and character evolution. Sinauer Associates, Sunderland, MA

    Google Scholar 

  • Muntanola-Cvetkovic M, Ristanovic B (1976) A new species of Embellisia isolated from seawater. Mycologia 68:47–51

    Article  Google Scholar 

  • Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14(9):817–818

    Article  PubMed  CAS  Google Scholar 

  • Pryor BM, Bigelow DM (2003) Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium. Mycologia 95:1141–1154

    Article  PubMed  CAS  Google Scholar 

  • Pryor BM, Gilbertson RL (2000) Molecular phylogenetic relationships amongst Alternaria species and related fungi based on analysis of nuclear ITS and mt SSU rDNA sequences. Mycol Res 104:1312–1321

    Article  CAS  Google Scholar 

  • Pryor BM, Michailides TJ (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92:406–416

    Article  PubMed  CAS  Google Scholar 

  • Pryor BM, Creamer R, Shoemaker RA, McLain-Romero J, Hambleton S (2009) Undifilum, a new genus of endophytic Embellisia oxytropis and Helminthosporium bornmuelleri on legumes. Bot 87:178–194

    Article  CAS  Google Scholar 

  • Rokas A, Williams BL, King N, Carroll BS (2003) Genome-scale approaches to resolving incongruency in molecular phylogenies. Nature 425:798–804

    Article  PubMed  CAS  Google Scholar 

  • Runa F, Park MS, Pryor BM (2009) Ulocladium systematics revisited: phylogeny and taxonomic status. Mycol Prog 8:35–47

    Article  Google Scholar 

  • Saccardo PA (1886) Sylloge Fungorum. Vol. IV. Padova: Publ by the author. p 807

  • Simmons EG (1967) Typification of Alternaria, Stemphylium, and Ulocladium. Mycologia 59:67–92

    Article  PubMed  CAS  Google Scholar 

  • Simmons EG (1971) Helminthosporium allii as type of a new genus. Mycologia 63:380–386

    Article  Google Scholar 

  • Simmons EG (1983) An aggregation of Embellisia species. Mycotaxon 17:216–241

    Google Scholar 

  • Simmons EG (1986) Alternaria themes and variations (22–26). Mycotaxon 25:287–308

    Google Scholar 

  • Simmons EG (1989) Macrospora Fuckel (Pleosporales) and related anamorphs. Sydowia 41:314–329

    Google Scholar 

  • Simmons EG (1990) Embellisia and related teleomorphs. Mycotaxon 38:251–265

    Google Scholar 

  • Simmons EG (1992) Alternaria taxonomy: current status, viewpoint, challenge. In: Chelkowski J, Visconti A (eds) Alternaria biology, plant diseases and metabolites. Elsevier Science Publishers, Amsterdam, pp 1–35

    Google Scholar 

  • Simmons EG (1995) Alternaria themes and variations (112–144). Mycotaxon 55:55–163

    Google Scholar 

  • Simmons EG (1997) Alternaria themes and variations (151–223). Mycotaxon 65:1–91

    Google Scholar 

  • Simmons EG (2000) Alternaria themes and variations (244–286): species on Solanaceae. Mycotaxon 75:1–115

    Google Scholar 

  • Simmons EG (2004) Novel dematiaceous hyphomycetes. Stud Mycol 50:109–118

    Google Scholar 

  • Simmons EG (2007) Alternaria an identification manual. CBS Fungal Biodiversity Centre, Utrecht, The Netherlands, pp 10–12

    Google Scholar 

  • Sivanesan A (1984) The Bitunicate Ascomycetes. J. Kramer, Vaduz, p 701

    Google Scholar 

  • Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts

    Google Scholar 

  • Tóth B, Csõsz M, Szabó-Hevér A, Simmons EG, Samson RA, Varga J (2011) Alternaria hungarica sp. Nov., a minor foliar pathogen of wheat in Hungary. Mycologia 103:94–100

    Article  PubMed  Google Scholar 

  • Wang Y, Pei Y, O’Neill NR, Zhang X (2010) Ulocladium cantlous sp. nov. isolated from northwestern China: its morphology and molecular phylogenetic position. Mycologia 102:374–383

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Geng Y, Ma J, Wang Q, Zhang X (2011) Sinomyces: a new genus of anamorphic Pleosporacea. Fungal Biology 115:188–195

    Article  PubMed  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. London: Academic Press, London, pp 315–322

  • Zhang Y, Schoch CL, Fournier J, Crous PW, de Gruyter J, Woudenberg JHC, Hirayama K, Tanaka K, Pointing SB, Spatafora JW, Hyde KD (2009) Multi-locus phylogeny of Pleosporales: a taxonomic, ecological and evolutionary re-evaluation. Stud Mycol 64:85–102

    Article  PubMed  CAS  Google Scholar 

  • Zhao GZ, Zhang TY (2005) Notes on dictyosporous hyphomycetes from China VII. The genus Nimbya Fungal Diversity 19:201–215

    Google Scholar 

  • van Zinderen Bakker EM (1940) Cercospora scirpicola (Sacc.) nov. comb. Revue de Mycol 5:64–69

    Google Scholar 

Download references

Acknowledgments

This work was supported in part by the University of Arizona College of Agriculture and Life Sciences, Tucson, and the National Science Foundation (DEB No. 0918758).

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Authors and Affiliations

  1. Division of Plant Pathology and Microbiology, Department of Plant Sciences, College of Agriculture, University of Arizona, Forbes 303, P.O. Box 210036, Tucson, AZ, 85721-0036, USA

    Daniel P. Lawrence, Myung Soo Park & Barry M. Pryor

  2. Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yusong, P.O. Box 107, Daejon, Korea

    Myung Soo Park

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  1. Daniel P. Lawrence
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  2. Myung Soo Park
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  3. Barry M. Pryor
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Correspondence to Barry M. Pryor.

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Lawrence, D.P., Park, M.S. & Pryor, B.M. Nimbya and Embellisia revisited, with nov. comb for Alternaria celosiae and A. perpunctulata . Mycol Progress 11, 799–815 (2012). https://doi.org/10.1007/s11557-011-0793-7

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  • DOI: https://doi.org/10.1007/s11557-011-0793-7

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