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Amyloidity is not diagnostic for species in the Mycena pearsoniana complex (Mycena sectio Calodontes)

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Abstract

In Mycena sectio Calodontes with otherwise amyloid spores, the inamyloid spores of Mycena pearsoniana Dennis ex Singer were a distinguishing feature for this species and its subsection Violacella. Although the original concept of this species was European, Singer chose to typify it with material collected in Mexico. The name has since been applied to all European collections with inamyloid spores and decurrent lamellae. Our phylogenetic analysis of 91 ITS sequences from European, North and South American Calodontes collections shows that European collections identified as M. pearsoniana fall into two well-supported sibling clades together with both inamyloid and weakly amyloid North American collections. Since the holotype of M. pearsoniana is in an advanced state of decay, we have selected an epitype from a North American locality with a climate comparable to the Mexican type locality. Our results show weakly and inamyloid spore reactions to be homoplastic in Calodontes, and furthermore that spores of M. pearsoniana can show either amyloid or inamyloid reactions interchangeably. This raises doubt about the taxonomic value of this trait in Mycena systematics.

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References

  • Aronsen A, (2011) A key to the Mycenas of Norway. http://home.online.no/~araronse/Mycenakey/whatsnew.htm

  • Brown JKM, Hovmøller MS (2002) Aerial dispersal ofpathogens on the global and continental scales and itsimpact on plant disease. Science 297:537–541

    Article  PubMed  CAS  Google Scholar 

  • Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552

    Article  PubMed  CAS  Google Scholar 

  • Cooke MC (1892) Handbook of Australian fungi. Williams and Norgate, London

  • Dennis RWG (1970) Fungus flora of Venezuela and adjacent countries. Kew Bulletin Additional Series 3. Royal Botanic Gardens, Kew

  • Frøslev TG, Jeppesen TS, Læssøe T, Kjøller R (2007) Molecular phylogenetics and delimitation of species in Cortinarius section Calochroi (Basidiomycota, Agaricales) in Europe. Mol Phyl Evol 44:217–227

    Article  Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118

    Article  PubMed  CAS  Google Scholar 

  • Gordon SA, Petersen RH (1997) Infraspecific variation among geographically separated collections of Marasmius androsaceus. Mycol Res 101:365–371

    Article  Google Scholar 

  • Gordon SA, Petersen RH (1998) Infraspecific variation among geographically separated collections of Marasmius scorodonius. Mycotaxon 69:453–466

    Google Scholar 

  • Grand EA, Hughes KW, Petersen RH (2010) Relationships within Lentinus subg. Lentinus (Polyporales, Agaricomycetes), with emphasis on sects. Lentinus and Tigrini. Mycol Prog (in press)

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Harder CB, Læssøe T, Kjøller R, Frøslev TG (2010) A comparison between ITS phylogenetic relationships and morphological species recognition within Mycena sect. Calodontes in Northern Europe. Mycol Prog 9:395–405

    Article  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  PubMed  CAS  Google Scholar 

  • Hughes KW, Petersen RH, Mata JL, Psurtseva NV, Kovalenko AE, Morozova OV, Lickey EB, Cifuentes Blanco J, Lewis DP, Nagasawa E, Halling RE, Takehashi S, Aime MC, Bau T, Henkel T (2007) Megacollybia (Agaricales). Report of the Tottori Mycological Institute 45:1–57

    Google Scholar 

  • Hughes KH, Petersen RH, Lickey EB (2009) Using heterozygosity to estimate a percentage DNA sequence similarity for environmental species’ delimitation across basidiomycete fungi. New Phytol 182:795–798

    Article  PubMed  CAS  Google Scholar 

  • James TY, Porter D, Hamrick JL, Vilgalys R (1999) Evidence for limited intercontinental gene flow in the cosmopolitan mushroom, Schizophyllum commune. Evolution 53:1665–1677

    Article  CAS  Google Scholar 

  • Jargeat P, Martos F, Carriconde F, Moreau PA, Gryta H, Gardes M (2010) Phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding: the case of the Tricholoma scalpturatum complex (Basidiomycota). Mol Ecol 19:5216–5320

    Article  PubMed  CAS  Google Scholar 

  • Jin J-K, Hughes KW, Petersen RH (2001) Biogeographical patterns in Panellus stypticus. Mycologia 93:309–316

    Article  CAS  Google Scholar 

  • Josserand M, 1960(”1959”). Notes critiques sur quelques champignons de la région lyonnaise (6 ser.). Bull Soc Mycol Fr 75:359–404

    Google Scholar 

  • Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518. doi:10.1093/nar/gki198

    Article  PubMed  CAS  Google Scholar 

  • Kornerup A, Wanscher JH (1978) Methuen handbook of colour, 3rd edn. Methuen, London

    Google Scholar 

  • Kühner R (1938) Genre Mycena (Fries). Étude cytologique et systématique des espèces d’Europe et d’Amérique du Nord. Encyclopédie mycologique, vol 10. Paul Lechévalier, Paris

  • Liang JF, Xu J, Yang ZL (2009) Divergence, dispersal and recombination in Lepiota cristata from China. Fungal Divers 38:105–24

    Google Scholar 

  • Lindner DL, Banik MT (2009) Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots. Mycologia 101:157–165

    Article  PubMed  CAS  Google Scholar 

  • Maas Geesteranus RA (1980) A tentative subdivision of the genus Mycena in the northern hemisphere. Studies in Mycenas − 15. Persoonia 11:93–120

    Google Scholar 

  • Maas Geesteranus RA (1989) Conspectus of the Mycenas of the Northern Hemisphere – 13. Sections Calamophilae and Calodontes. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen 92(4):477–504

    Google Scholar 

  • Matheny PB, Curtis JC, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjarden DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS (2006) Major clades of Agaricales: a multi-locus phylogenetic overview. Mycologia 98:982–995

    Article  PubMed  Google Scholar 

  • Nagarajan S, Singh DV (1990) Long distance dispersal of rust pathogens. Annu Rev Phytopathol 28:139–153

    Article  PubMed  CAS  Google Scholar 

  • Nylander JAA 2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University

  • Pearson AA (1955) Mycena. The Naturalist, April-June, 41–63

  • Pegler DN (1983) Agaric flora of the Lesser Antilles. Kew Bulletin, Additional Series IX. Royal Botanic Gardens, Kew

  • Pringle A, Adams RI, Cross HB, Bruns TD (2009) The ectomycorrhizal fungus Amanita phalloides was introduced and is expanding its range on the west coast of North America. Mol Ecol 18:817–833

    Article  PubMed  CAS  Google Scholar 

  • Queloz V, Sieber TN, Holdenrieder O, McDonald B, Grünig CR (2011) No biogeographical pattern for a root-associated fungal species complex. Glob Ecol Biogeogr 20:160–169

    Article  Google Scholar 

  • Rambaut A, Drummond, AJ (2007) Tracer v1.4. http://evolve.zoo.ox.ac.uk/

  • Rexer KH (1994) Die Gattung Mycena s. l. - Studien zu ihrer Anatomie, Morphologie und Systematik. Dissertation, Universität Tübingen

  • Ridgway R (1912) Color Standards and Color Nomenclature. Washington, D.C., published privately (by the author)

  • Robich G (2003) Mycena d’Europa. A.M.B., Fondazione Centro Studi Micologici. Trento, Vicenza

  • Singer R (1959)"1958" Fungi mexicani, series secunda Agaricales. Sydowia 12:221–243

    Google Scholar 

  • Smith AH (1947) North American species of Mycena. University of Michigan Press, Ann Arbor

    Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (and Other Methods) Version 4.10. Sinauer, Sunderland, MA

  • Taylor AFS (2008) Recent advances in our understanding of fungal ecology. Coolia 51:197–212

    Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 31:21–32

    Article  PubMed  CAS  Google Scholar 

  • Taylor JW, Turner E, Townsend JP, Dettman JR, Jacobson D (2006) Eukaryotic microbes, species recognition and the geographic limits of species: examples from the kingdom Fungi. Philos Trans R Soc Lond B 361:1947–1963

    Article  Google Scholar 

  • Vellinga EC, Wolfe BE, Pringle A (2009) Global patterns of ectomycorrhizal introductions. New Phytol 181:960–97\3

    Article  PubMed  Google Scholar 

  • Vilgalys R, Sun BL (1994) Ancient and recent patterns of geographic speciation in the oyster mushroom Pleurotus revealed by phylogenetic analysis of ribosomal DNA sequences. Proc Nat Acad Sci USA 91:4599–4603

    Article  PubMed  CAS  Google Scholar 

  • Wilgenbusch JC, Warren DL, Swofford DL (2004) AWTY: A system for graphical exploration of MCMC convergence in Bayesian phylogenetic inference. http://ceb.csit.fsu.edu/awt

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Acknowledgements

We thank the curator of the LILOA herbarium in Argentina for loaning the holotype of M. pearsoniana for study. Collecting in the Great Smoky Mountain National Park was supported by US National Science Foundation grant DBI 6338699 to K.W. Hughes and R.H. Petersen at the University of Tennesse, Knoxville. C.B. Harder was supported by The Danish Council for Strategic Research grant (2104-08-0012, MIRESOWA) at the time of the writing. The authors thank B.A. Perry and the following pre-reviewers for valuable comments on previous versions of the manuscript: K. O’Donnell, J. Geml and D.E. Desjardin.

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Authors and Affiliations

  1. Department of Biology, Terrestrial Ecology, University of Copenhagen, Øster Farimagsgade 2D, 1353, Copenhagen K, Denmark

    Christoffer Bugge Harder

  2. Center for Forest Mycology Research, USDA-Forest Service, Northern Research Station, PO Box 1377, Luquillo, PR, 00773-1377, USA

    D. Jean Lodge

  3. The University of Tennessee, 437 Hesler Biology Building, Knoxville, TN, 37996-1100, USA

    Ronald H. Petersen & Karen W. Hughes

  4. Facultad de Ciencias, UNAM, Circuito Exterior, Ciudad Universitaria, Mexico, D.F.E0, 4510, Mexico

    Joaquin Cifuentes Blanco

  5. Laboratoriet Filadelfia, Kolonivej 11, 4293, Dianalund, Denmark

    Tobias Guldberg Frøslev

  6. Department of Biology, Ecology and Evolution, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen Ø, Denmark

    Thomas Læssøe

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  1. Christoffer Bugge Harder
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Correspondence to Christoffer Bugge Harder.

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This paper was prepared by a US Government employee as part of their official duties and it is therefore in the public domain and not subject to copyright.

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Harder, C.B., Lodge, D.J., Petersen, R.H. et al. Amyloidity is not diagnostic for species in the Mycena pearsoniana complex (Mycena sectio Calodontes) . Mycol Progress 11, 725–732 (2012). https://doi.org/10.1007/s11557-011-0782-x

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