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Characterization of a Plasmopara isolate from Helianthus × laetiflorus based on cross infection, morphological, fatty acids and molecular phylogenetic data

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Abstract

A collection of Plasmopara halstedii s. l. from Helianthus × laetiflorus is recorded, described and characterised by laboratory cross infection studies, light microscopical studies, fatty acid pattern and LSU rDNA data. Its ability to infect Helianthus annuus was demonstrated in laboratory cross infection studies, however, it could not be unequivocally ascribed to a known pathotype. Microscopically, it shows no differences from isolates originating from Helianthus annuus, its fatty acid pattern is indistinguishable, and also its LSU rDNA sequence is identical. Due to this identity in a broad range of characters, the investigated downy mildew isolates from H. × laetiflorus and from H. annuus are regarded as conspecific.

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References

  • Anonymous (1966) Index of fungi 3(12), p. 364. Commonwealth Mycological Institute, Kew.

    Google Scholar 

  • Delanoe D (1972) Biologie et epidemiologie du mildiou du tournesol. CETIOM Informations Techniques 26: 1–61.

    Google Scholar 

  • Berlese AN, De Toni JB (1888) Phycomyceteae. In Saccardo PA (ed.) Sylloge Fungorum Omnium Hucusque Cognitorum, vol 7, pp. 181–322.

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. — Evolution 39: 783–791.

    Google Scholar 

  • Gu X, Fu YX, Li WH (1995) Maximum likelihood estimation of the heterogeneity of substitution rate among nucleotide sites. — Molecular Biology and Evolution 12: 546–557.

    CAS  PubMed  Google Scholar 

  • Gulya TJ, Rashid KY, Masirevic SM (1997) Sunflower diseases. In Schneiter AA (ed.) Sunflower technology and production, pp. 263–379. No 35 Agronomy. American Society of Agronomy, Madison, Wisconsin, USA.

    Google Scholar 

  • Gulya TJ, Tourvieille de Labrouhe D, Masirevic SM, Penaud A, Rashid KY, Viranyi F (1998) Proposal for standardized nomenclature and identification of races of Plasmopara halstedii (sunflower downy mildew). In Proceedings of the ISA Symposium. III. Sunflower Downy Mildew. Fargo, ND, 13–14 Jan. 1998, pp. 130–136.

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. — Nucleic Acids Symposium Series 41: 95–98.

    CAS  Google Scholar 

  • Hasegawa M, Kishino H, Yano T (1985) Dating of the human-ape splitting by a molecular clock of mitchondrial DNA. — Journal of Molecular Evolution 22: 160–174.

    CAS  PubMed  Google Scholar 

  • Leppik EE (1966) Origin and specialization of Plasmopara halstedii complex on the Compositae. — FAO Plant Production Bulletin 14: 72–76.

    Google Scholar 

  • Moncalvo JM, Wang HH, Hseu RS (1995) Phylogenetic relationships in Ganoderma inferrred from the internal transcribed spacers and 25S ribosomal DNA sequences. — Mycologia 87: 223–238.

    CAS  Google Scholar 

  • Nishimura M (1922) Studies in Plasmopara halstedii. — Journal of the College of Agriculture, Hokkaido Imperial University 11: 185–210.

    Google Scholar 

  • Novotelnova NS (1962a) K woprosu o ložnoj mučnistoj rose podsolnečnika. — Sbornik Dokladov Naučnoj Konferencii po Zaščita Rastenij (Tallin): 129–139.

  • Novotelnova NS (1962b) Plasmopara halstedii (Farl.) Berl. et De Toni as a conspecies (substantiation of the taxonomic classification of the species of Plasmopara infesting Compositae). — Botaničeskij Žurnal 47: 970–981 (in Russian).

    Google Scholar 

  • Novotelnova NS (1963) Nowye widy Plasmopara na Compositae. — Botaničeskie materialy Otdela sporvykh rastenii Botanicheskogo instituta Akademii nauk SSSR 16: 72–83.

    Google Scholar 

  • O’Donnell K (1993). Fusarium and its near relatives. In Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics, pp. 225–233. CAB International, Wallingford, UK.

    Google Scholar 

  • Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. — Bioinformatics 14: 817–818.

    Article  CAS  PubMed  Google Scholar 

  • Riethmüller A, Voglmayr H, Göker M, Weiß M, Oberwinkler F (2002) Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. — Mycologia 94: 834–849.

    Google Scholar 

  • Rozynek B, Spring O (2000) Pathotypes of sunflower downy mildew in southern parts of Germany. — Helia 23: 27–34.

    Google Scholar 

  • Sackston WE (1981) Downy mildew of sunflower. In Spencer DM (ed.) The Downy Mildews, pp. 545–575. Academic Press, London.

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. — Molecular Biology and Evolution 4: 406–425.

    CAS  PubMed  Google Scholar 

  • Spring O, Haas K (2002a) The fatty acid composition of Plasmopara halstedii and its taxonomic significance. — European Journal of Plant Pathology 108: 263–267.

    Article  CAS  Google Scholar 

  • Spring O, Haas K (2002b) The taxonomic significance of fatty acid patterns in biotrophic Oomycetes. — Proceedings Botanikertagung 2002, Freiburg, Germany, Deutsche Botanische Gesellschaft, p. 436.

    Google Scholar 

  • Spring O, Rozynek B, Zipper R (1997) Leaf disk inoculation-a useful tool for selecting infections of sunflower downy mildew at low inoculum concentration, but inappropriate to pathotypes characterization. — Journal of Phytopathology 145: 189–191.

    Google Scholar 

  • Spring O, Schilling EE (1990) The origin of Helianthus × multiflorus and H. × laetiflorus (Asteraceae). — Biochemical Systematics and Ecology 18: 19–23.

    CAS  Google Scholar 

  • Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), ver. 4b10. Sinauer, Sunderland, Mass.

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. — Nucleic Acids Research 24: 4876–4882.

    Google Scholar 

  • Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. — Journal of Bacteriology 172: 4238–4246.

    CAS  PubMed  Google Scholar 

  • Virányi F (1984) Recent research on the downy mildew of sunflower in Hungary. — Helia 7: 35–38.

    Google Scholar 

  • Walcz I, Bogar K, Viranyi F (2000) Study on an Ambrosia isolate of Plasmopara halstedii. — Helia 23: 19–24.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Botanik, Universität Hohenheim, Garbenstr. 30, D-70593, Stuttgart, Germany

    Otmar Spring

  2. Institut für Botanik, Universität Wien, Rennweg 14, A-1030, Wien, Austria

    Hermann Voglmayr

  3. Lehrstuhl für Spezielle Botanik und Mykologie, Botanisches Institut, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany

    Alexandra Riethmüller & Franz Oberwinkler

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  1. Otmar Spring
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  2. Hermann Voglmayr
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  3. Alexandra Riethmüller
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  4. Franz Oberwinkler
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Correspondence to Otmar Spring.

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Spring, O., Voglmayr, H., Riethmüller, A. et al. Characterization of a Plasmopara isolate from Helianthus × laetiflorus based on cross infection, morphological, fatty acids and molecular phylogenetic data. Mycol Progress 2, 163–170 (2003). https://doi.org/10.1007/s11557-006-0054-3

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  • DOI: https://doi.org/10.1007/s11557-006-0054-3

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