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ITS rDNA sequence-based phylogenetic analysis of Tomentellopsis species from boreal and temperate forests, and the identification of pink-type ectomycorrhizas

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Abstract

Fungi in the genus Tomentellopsis were subjected to molecular phylogenetic analyses in order to clarify species-level relationships and mycorrhiza-forming ability with coniferous and deciduous trees. Fungal nucleotide sequence data from the internal transcribed spacer of nuclear ribosomal DNA (ITS rDNA) region were obtained from fruitbodies, ectomycorrhiza and pure cultures. Maximum parsimony (MP), distance (neighbor joining, NJ) and maximum likelihood (ML) analyses of aligned ITS sequences highlighted three clades designated T. echinospora, T. submollis and T. bresadoliana. Sporocarp tissue and ectomycorrhizas, or isolated mycelia, previously described as Pinirhiza rosea, Piceirhiza rosea or Pink were clearly identified in a strongly supported T. submollis clade. Host-linked population variation in this clade was also noted that may reflect active speciation activity. A fungus isolated from Beige-type mycorrhizas formed on Scots pine seedlings appeared in the paraphyletic T. echinospora clade which included sequences showing greater divergence. The data provide a primary classification of Tomentellopsis species that is urgently needed in assessment of the importance of resupinate thelephoroid fungi in mycorrhizal communities associated with trees in coniferous and deciduous forest ecosystems.

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Authors and Affiliations

  1. Institute of Botany and Ecology, University of Tartu, 40 Lai St., 51005, Tartu, Estonia

    Urmas Kõljalg

  2. Department of Biosciences, Division of General Microbiology, Viikki Biocenter, University of Helsinki, P.O. Box 56, FIN-00014, Finland

    Hanna Tammi, Sari Timonen & Robin Sen

  3. Institute for Systematic Botany, Section Mycology, University of München, Menzinger Str. 67, D-80638, München, Germany

    Reinhard Agerer

  4. Institute of Zoology and Botany, Estonian Agricultural University, Estonia

    Urmas Kõljalg

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  1. Urmas Kõljalg
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  2. Hanna Tammi
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  4. Reinhard Agerer
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  5. Robin Sen
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Correspondence to Urmas Kõljalg.

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Kõljalg, U., Tammi, H., Timonen, S. et al. ITS rDNA sequence-based phylogenetic analysis of Tomentellopsis species from boreal and temperate forests, and the identification of pink-type ectomycorrhizas. Mycol Progress 1, 81–92 (2002). https://doi.org/10.1007/s11557-006-0007-x

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