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Short-term glucagon stimulation test of C-peptide effect on glucose utilization in patients with type 1 diabetes mellitus

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An Erratum to this article was published on 14 January 2016

Abstract

This work aimed to evaluate the use of a four-point glucagon stimulation test of C-peptide effect on glucose utilization in type 1 diabetic patients using a new mathematical model. A group of 32 type 1 diabetic patients and a group of 10 healthy control subjects underwent a four-point glucagon stimulation test with blood sampling at 0, 6, 15 and 30 min after 1 mg glucagon bolus intravenous administration. Pharmacokinetic and pharmacokinetic/pharmacodynamic models of C-peptide effect on glucose utilization versus area under curve (AUC) were used. A two-sample t test and ANOVA with Bonferroni correction were used to test the significance of differences between parameters. A significant difference between control and patient groups regarding the coefficient of whole-body glucose utilization and AUC C-peptide/AUC glucose ratio (p ≪ 0.001 and p = 0.002, respectively) was observed. The high correlation (r = 0.97) between modeled coefficient of whole-body glucose utilization and numerically calculated AUC C-peptide/AUC glucose ratio related to entire cohort indicated the stability of used method. The short-term four-point glucagon stimulation test allows the numerically calculated AUC C-peptide/AUC glucose ratio and/or the coefficient of whole-body glucose utilization calculated from model to be used to diagnostically identify type 1 diabetic patients.

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Acknowledgments

The authors would like to thank the reviewers for valuable and insightful comments and reviewing that helped improve the manuscript. This publication was supported by the Competence Centre for SMART Technologies for Electronics and Informatics Systems and Services, ITMS 26240220072, funded by the Research & Development Operational Program of the ERDF, Scientific Grant Agency VEGA 1/0338/08, and by the Philanthropic fund of Studio 727, Bratislava.

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Authors and Affiliations

  1. IIIrd Department of Internal Medicine, Derer’s University Hospital, Limbova 5, 833 05, Bratislava, Slovakia

    Viliam Mojto

  2. Institute of Automation, Measurement and Applied Informatics, Faculty of Mechanical Engineering, Slovak University of Technology, Namestie slobody 17, 812 31, Bratislava, Slovakia

    Zuzana Rausova, Jana Chrenova & Ladislav Dedik

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  1. Viliam Mojto
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  2. Zuzana Rausova
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Correspondence to Jana Chrenova.

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The authors disclose that there are no actual or potential conflicts of interest including any financial, personal or other relationships with other people or organizations that could inappropriately influence (bias) their work.

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Mojto, V., Rausova, Z., Chrenova, J. et al. Short-term glucagon stimulation test of C-peptide effect on glucose utilization in patients with type 1 diabetes mellitus. Med Biol Eng Comput 53, 1361–1369 (2015). https://doi.org/10.1007/s11517-015-1416-2

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