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Biological Effect and Inactivation Mechanism of Bacillus subtilis Exposed to Pulsed Magnetic Field: Morphology, Membrane Permeability and Intracellular Contents

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Abstract

Biological effect and inactivation mechanism of Bacillus subtilis exposed to pulsed magnetic field (PMF) were investigated. Cell morphology, membrane permeability, release of intracellular proteins and damage of deoxyribonucleic acid (DNA) were monitored. The inactivation effect of PMF treatment on B. subtilis was enhanced with the increase in intensity and pulse number. Scanning electron microscopy (SEM) analysis showed that the PMF treated bacterial cells had rough surfaces and damaged cellular membranes. Flow cytometry measurements showed that the cell membrane permeability increased after the PMF treatment. Leakage of intracellular contents, measured as OD, significantly increased with the increase in PMF intensity and pulsed number. A maximum leakage of cytoplasmic contents was detected at intensity of 3.30 T and 30 pulses. Enterobacterial repetitive intergenic consensus - polymerase chain reaction (ERIC-PCR) fingerprint signaled that the DNA was fragmented after the PMF treatment at the intensity of 3.0 T with 30 pulses and 3.3 T with 10, 20 and 30 pulses. These findings may give clues to the mechanism of bacterial cell death due to the PMF treatment.

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Acknowledgments

The authors wish to thank the National Natural Science Foundation of China (No. 31271966), National Key Research and Development Program (No. 2016YFD0400700-05), Natural Science Foundation of Jiangsu Province (No. BK20150498), The social development project of Jiangsu Science and Technology Department (No. BE2016740834) and Research and Innovation Project for Postgraduate of Higher Education Institutions of Jiangsu Province (No. CX10B-021X) for their valuable support.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, No.301 Xuefu Road, Zhenjiang, 212013, China

    Jingya Qian, Cunshan Zhou, Haile Ma, Shujun Li & Mohammed A. Y. Abdualrahman

  2. Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, Jiangsu University, No.301 Xuefu Road, Zhenjiang, 212013, China

    Jingya Qian, Cunshan Zhou & Haile Ma

  3. Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing, 334221, China

    Jingya Qian

  4. Chinese Academy of Agricultural Mechanization Sciences, No.1 Beishatan Deshengmen Wai, Chaoyang District, Beijing, 100083, China

    Shujun Li

  5. Faculty of Agriculture, University of Zalingie, PO Box 6, Zalingie, Sudan

    Abu ElGasim A. Yagoub

  6. Department of Food Technology, Nyala Technical College, Southern Darfur State, Nyala, Sudan

    Mohammed A. Y. Abdualrahman

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  1. Jingya Qian
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  2. Cunshan Zhou
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  3. Haile Ma
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  5. Abu ElGasim A. Yagoub
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Correspondence to Haile Ma.

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Jingya Qian and Cunshan Zhou are joint first author

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Qian, J., Zhou, C., Ma, H. et al. Biological Effect and Inactivation Mechanism of Bacillus subtilis Exposed to Pulsed Magnetic Field: Morphology, Membrane Permeability and Intracellular Contents. Food Biophysics 11, 429–435 (2016). https://doi.org/10.1007/s11483-016-9442-7

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  • DOI: https://doi.org/10.1007/s11483-016-9442-7

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