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Thermal Stability of the Iron–Lactoferrin Complex in Aqueous Solution is Improved by Soluble Soybean Polysaccharide

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Abstract

Lactoferrin (Lf) can solubilize more than a 70-fold molar equivalent of iron in the presence of bicarbonate anions. The resulting iron–Lf complex (FeLf) is a useful food ingredient for iron fortification to prevent anemia. Although FeLf has greater thermal stability than Lf, a pasteurizing technique for FeLf has not been established. The aim of the present study was to develop a practical technique to pasteurize FeLf based on its thermal stability with the aid of a polysaccharide. FeLf [0.1 %, weight/weight (w/w) ratio] was heated at 80 °C for 3 min under various pH (5.5–8.0) and electrical conductivity (0.1–6.0 mS/cm) levels. Overall, FeLf was thermally stable and was hardly affected by pH or electrical conductivity, although aggregation and precipitation occurred when FeLf was heated at pH 6.0–7.5 in the presence of salt and electrical conductivity >3.0 mS/cm. When 0.01 %–0.4 % (w/w) of soluble soybean polysaccharide (SSPS) was added to 0.01 % (w/w) FeLf solution, the FeLf remained soluble and maintained its iron-holding property at pH 6.5, even when heated at 120 °C for 4 min. Particle charge measurements showed that the ζ-potential of FeLf-SSPS became negatively charged following the addition of SSPS, which was associated with the improved thermal stability of FeLf. These results have important implications for the use of FeLf in developing liquid-based food products.

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Abbreviations

Lf:

Lactoferrin

FeLf:

Iron–lactoferrin complex

SSPS:

Soluble soybean polysaccharide

FeLf-SSPS:

Mixture of FeLf and SSPS

References

  1. World Health Organization, Worldwide prevalence of anaemia 1993–2005: World Health Organization global database on anaemia. (World Health Organization, 2008), http://whqlibdoc.who.int/publications/2008/9789241596657_eng.pdf. Accessed March 21, 2012

  2. R.F. Hurrell, Preventing iron deficiency through food fortification. Nutr. Rev. 55, 210–222 (1997)

    Article  CAS  Google Scholar 

  3. T. Waraho, D.J. McClements, E.A. Decker, Mechanisms of lipid oxidation in food dispersions. Trends Food Sci. Technol. 22, 3–13 (2011)

    Article  CAS  Google Scholar 

  4. J.M. Steijns, A.C. van Hooijdonk, Occurrence, structure, biochemical properties and technological characteristics of lactoferrin. Br. J. Nutr. 84, S11–S17 (2000)

    Article  CAS  Google Scholar 

  5. H. Wakabayashi, K. Yamauchi, M. Takase, Lactoferrin research, technology and applications. Int. Dairy J. 16, 1241–1251 (2006)

    Article  CAS  Google Scholar 

  6. B. Lönnerdal, Nutritional roles of lactoferrin. Curr. Opin. Clin. Nutr. Metab. Care 12, 293–297 (2009)

    Article  Google Scholar 

  7. Y. Nagasako, H. Saito, Y. Tamura, S. Shimamura, M. Tomita, Iron-binding properties of bovine lactoferrin in iron-rich solution. J. Dairy Sci. 76, 1876–1881 (1993)

    Article  CAS  Google Scholar 

  8. E.N. Baker, H.M. Baker, A structural framework for understanding the multifunctional character of lactoferrin. Biochimie 91, 3–10 (2009)

    Article  CAS  Google Scholar 

  9. H. Kawakami, S. Dosako, I. Nakajima, Effect of lactoferrin on iron solubility under neutral conditions. Biosc. Biotechnol. Biochem. 57, 1376–1377 (1993)

    Article  CAS  Google Scholar 

  10. H.M. Ueno, K. Kato, N. Ueda, H. Matsui, H. Nakajima, Native, but not thermally denatured lactoferrin solubilizes iron in the presence of bicarbonate ions. Dairy Sci. Technol. 92, 25–35 (2012)

    Article  CAS  Google Scholar 

  11. T. Uchida, T. Oda, K. Sato, H. Kawakami, Availability of lactoferrin as a natural solubilizer of iron for food products. Int. Dairy J. 16, 95–101 (2006)

    Article  CAS  Google Scholar 

  12. M. Shiota, T. Uchida, T. Oda, H. Kawakami, Utilization of lactoferrin as an iron-stabilizer for soybean and fish oil. J. Food Sci. 71, C120–C123 (2006)

    Article  CAS  Google Scholar 

  13. F. Hu, F. Pan, Y. Sawano, T. Makino, Y. Kakehi, M. Komiyama, H. Kawakami, M. Tanokura, Studies of the structure of multiferric ion-bound lactoferrin: A new antianemic edible material. Int. Dairy J. 18, 1051–1056 (2008)

    Article  CAS  Google Scholar 

  14. H. Abe, H. Saito, H. Miyakawa, Y. Tamura, S. Shimamura, E. Nagao, M. Tomita, Heat stability of bovine lactoferrin at acidic pH. J. Dairy Sci. 74, 65–71 (1991)

    Article  CAS  Google Scholar 

  15. H. Kawakami, M. Tanaka, K. Tatsumi, S. Dosako, Effects of ionic strength and pH on the thermostability of lactoferrin. Int. Dairy J. 2, 287–298 (1992)

    Article  CAS  Google Scholar 

  16. S.L. Turgeon, C. Schmitt, C. Sanchez, Protein–polysaccharide complexes and coacervates. Curr. Opin. Coll. Interface Sci. 12, 166–178 (2007)

    Article  CAS  Google Scholar 

  17. C. Bengoechea, O.G. Jones, A. Guerrero, D.J. McClements, Formation and characterization of lactoferrin/pectin electrostatic complexes: Impact of composition, pH and thermal treatment. Food Hydrocoll. 25, 1227–1232 (2011)

    Article  CAS  Google Scholar 

  18. A. Nakamura, H. Furuta, H. Maeda, T. Takao, Y. Nagamatsu, Structural studies by stepwise enzymatic degradation of the main backbone of soybean soluble polysaccharides consisting of galacturonan and rhamnogalacturonan. Biosci. Biotechnol. Biochem. 66, 1301–1313 (2002)

    Article  CAS  Google Scholar 

  19. H. Maeda, in Handbook of Hydrocolloids, ed. by G.O. Phillips, P.A. Williams (Woodhead Publishing, Cambridge, 2000), p. 309

    Google Scholar 

  20. M. Nakamura, T. Funami, S. Noda, S. Ishihara, S. Al-Assaf, K. Nishinari, G.O. Philips, Comparison of sugar beet pectin, soybean soluble polysaccharide, and gum Arabic as food emulsifiers. 1. Effect of concentration, pH, and salts on the emulsifying properties. Food Hydrocoll 22, 1254–1267 (2008)

    Article  Google Scholar 

  21. N. Fafaungwithayakul, P. Hongsprabhas, P. Hongsprabhas, Effect of soy soluble polysaccharide on the stability of soy-stabilised emulsions during in vitro protein digestion. Food Biophys. 6, 407–415 (2011)

    Article  Google Scholar 

  22. A. Nakamura, R. Yoshida, H. Maeda, M. Corredig, The stabilizing behavior of soybean soluble polysaccharide and pectin in acidified milk beverages. Int. Dairy J. 16, 361–369 (2006)

    Article  CAS  Google Scholar 

  23. H. Kawakami, H. Shinmoto, S. Dosako, Y. Sogo, One-step isolation of lactoferrin using immobilized monoclonal antibodies. J. Dairy Sci. 70, 752–759 (1987)

    Article  CAS  Google Scholar 

  24. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)

    Article  CAS  Google Scholar 

  25. M. Sugiarto, A. Ye, M.W. Taylor, H. Singh, Milk protein–iron complexes: Inhibition of lipid oxidation in an emulsion. Dairy Sci. Technol. 90, 87–98 (2010)

    Article  CAS  Google Scholar 

  26. I. Peinado, U. Lesmes, A. Andrés, D.J. McClements, Fabrication and morphological characterization of biopolymer particles formed by electrostatic complexation of heat treated lactoferrin and anionic polysaccharides. Langmur 26, 9827–9834 (2010)

    Article  CAS  Google Scholar 

  27. S. Noda, T. Funami, M. Nakauma, I. Asai, R. Takahashi, S. Al-Assaf, S. Ikeda, K. Nishinari, G.O. Phillips, Molecular structures of gellangum imaged with atomic force microscopy in relation to the rheological behavior in aqueous systems. 1. Gellangum with various acyl contents in the presence and absence of potassium. Food Hydrocoll 22, 1148–1159 (2008)

    Article  CAS  Google Scholar 

  28. A. Dartois, J. Singh, L. Kaur, H. Singh, Influence of guar gum on the in vitro starch digestibility—rheological and microstructural characteristics. Food Biophys. 5, 149–160 (2010)

    Article  Google Scholar 

  29. M.A.S.A. Samir, F. Alloin, A. Dufresne, Review of recent research into cellulosic whiskers, their properties and their application in nanocomposite field. Biomacromolecules 6, 612–626 (2005)

    Article  CAS  Google Scholar 

  30. Ministry of Health, Labour and Welfare, Ordinance for Enforcement of the Food Sanitation Act. (Ministry of Health, Labour, and Welfare, 2007), p. 15, http://www.cas.go.jp/jp/seisaku/hourei/data/oefs.pdf. Accessed May 1, 2012

  31. United States Department of Agriculture, What we eat in America, NHANES 2007–2008, individuals 2 years and over (excluding breast-fed children), day 1 dietary intake data, weighted. (United States Department of Agriculture, 2010), http://www.ars.usda.gov/SP2UserFiles/Place/12355000/pdf/0708/Table_1_NIN_GEN_07.pdf. Accessed August 10, 2010

  32. United States Department of Agriculture, Dietary Reference Intakes: Recommended Intakes for Individuals. (United States Department of Agriculture, 2010), http://www.iom.edu/Activities/Nutrition/SummaryDRIs/~/media/Files/Activity%20Files/Nutrition/DRIs/5_Summary%20Table%20Tables%201-4.pdf. Accessed September 13, 2011

  33. European Food Safety Authority, Tolerable upper intake levels for vitamins and minerals by the scientific panel on dietetic products, nutrition and allergies and scientific committee on food. (European Food Safety Authority, 2006), http://www.efsa.europa.eu/en/ndatopics/docs/ndatolerableuil.pdf. Accessed February 12, 2012

  34. Ministry of Health, Labour and Welfare, National health and nutrition survey in Japan, 2009. (Ministry of Health, Labour, and Welfare, 2012), http://www.mhlw.go.jp/bunya/kenkou/eiyou/dl/h21-houkoku-07.pdf. Accessed 7 December 7, 2010

  35. Ministry of Health, Labour, and Welfare, Dietary reference intakes for Japanese (2010). (Ministry of Health, Labour, and Welfare, 2009), http://www.mhlw.go.jp/shingi/2009/05/dl/s0529-4ah.pdf. Accessed May 29, 2009

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Acknowledgments

We would like to thank Prof. Hirokazu Matsui (Graduate School of Agriculture, Hokkaido University, Japan) for his insightful comments and suggestions.

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Authors and Affiliations

  1. Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, Kawagoe, Saitama, 350-1165, Japan

    Hiroshi M. Ueno, Noriko Ueda, Momoko Morita, Yuji Kakehi & Toshiya Kobayashi

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  1. Hiroshi M. Ueno
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  2. Noriko Ueda
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  3. Momoko Morita
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  5. Toshiya Kobayashi
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Correspondence to Toshiya Kobayashi.

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Ueno, H.M., Ueda, N., Morita, M. et al. Thermal Stability of the Iron–Lactoferrin Complex in Aqueous Solution is Improved by Soluble Soybean Polysaccharide. Food Biophysics 7, 183–189 (2012). https://doi.org/10.1007/s11483-012-9256-1

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