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LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis

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Abstract

The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocin-induced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p < 0.001) and 18.6% (p < 0.01), respectively. However, the E. officinalis seed extract treatment showed marked improvements in CAT and SOD activities by 47.09% (p < 0.001) and 21.61% (p < 0.001), respectively. The enhanced lipid peroxidation by 30.87% (p < 0.001) in erythrocytes of untreated diabetic rats was significantly accentuated in the extract treated animals by 23.72% (p < 0.001). The erythrocytes showed increased osmotic fragility due to diabetes in terms of hemolysis. It attained the normal level in diabetic treated group. The findings thus suggest that E. officinalis seed extract has the potential to be exploited as an agent to boost the antioxidant system in the diabetic animal model. Laser-induced breakdown spectroscopy has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca (II), Mg (II) and Fe (II) as reflected by their intensities are responsible for the antioxidant potential of E. officinalis.

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Acknowledgments

The authors are grateful to BRNS, BARK Mumbai, India, for providing the financial assistance. First author Shikha Mehta is thankful to University Grants Commission (UGC), New Delhi, India for the award of SRF.

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Authors and Affiliations

  1. Alternative Therapeutics Unit, Drug Discovery & Development Division, Medicinal Research Lab, Department of Chemistry, University of Allahabad, Allahabad, 211002, India

    Shikha Mehta, Prashant Kumar Rai & Geeta Watal

  2. Department of Biochemistry, University of Allahabad, Allahabad, 211002, India

    Devendra Kumar Rai & Bechan Sharma

  3. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, 211002, India

    Nilesh Kumar Rai & A. K. Rai

  4. Laboratory of Biophysics, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenlaan 3-Transitorium, 6703 HA, Wageningen, The Netherlands

    Dane Bicanic

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  1. Shikha Mehta
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  2. Prashant Kumar Rai
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Correspondence to Geeta Watal.

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Mehta, S., Rai, P.K., Rai, D.K. et al. LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis . Food Biophysics 5, 186–192 (2010). https://doi.org/10.1007/s11483-010-9158-z

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