Abstract
The kinetics of phase separation and microstructure of oat β-glucan/whey protein binary mixtures varying in concentration (4–16% w/v protein, 0.3–1.2% w/v β-glucan) and β-glucan molecular weight (1.3 × 106, 640 × 103, 180 × 103, and 120 × 103 g/mol) was investigated by turbidimetry and fluorescent microscopy. The phase separation of the mixed systems was followed at pH 7.0 and at room temperature under quiescent conditions. Application of first principles revealed that phase separation of the systems follows first-order kinetics. Acceleration of the phase-separation process was observed with increase of β-glucan concentration for the three lowest-MW samples but the highest molecular weight (1.3 × 106 g/mol) exhibited the opposite trend. Changes in the polysaccharide molecular weight resulted in considerable differences in β-glucan aggregate morphology in the mixed systems. The change in the continuity of the mixed system from polysaccharide-, to bi-, to protein-continuous was confirmed for a wide range of mixed systems differing in biopolymer concentration, and β-glucan molecular weight.
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A. Lazaridou, C.G. Biliaderis, J. Cereal Sci. 46, 101 (2007)
P.J. Wood, J. Cereal Sci. 46, 230 (2007)
J.T. Braaten, F.W. Scott, P.J. Wood, K.D. Riedel, M.S. Wolynetz, D. Brulé, M.W. Collins, Diabetes Med. 11, 312 (1994)
J.T. Braaten, P.J. Wood, F.W. Scott, M.S. Wolynetz, M.K. Lowe, P. Bradley-White, M.W. Collins, J. Clin, Nutr. 48, 465–474 (1994)
X. Lan-Pidhainy, Y. Brummer, S.M. Tosh, T.M. Wolever, P.J. Wood, Cereal Chem. 84, 512 (2007)
K.M. Behall, D.J. Scholfield, J. Hallfrisch, Am. J. Clin. Nutr. 80, 1185 (2004)
S.M. Tosh, Y. Brummer, T.M. Wolever, P.J. Wood, Cereal Chem. 85, 211 (2008)
FDA, Fed. Regist. 15, 3584 (1997)
P.F. Fox, P.L.H. McSweeney, Advanced dairy chemistry, volume 1—proteins, 3rd edn. (Kluwer, New York, 2003)
A. Lazaridou, C.G. Biliaderis, Food Hydrocoll. 23, 886 (2009)
A. Lazaridou, H. Vaikousi, C.G. Biliaderis, Int. Dairy J. 18, 312 (2008)
V. Kontogiorgos, C. Ritzoulis, C.G. Biliaderis, S. Kasapis, Food Hydrocoll. 20, 749 (2006)
V. Kontogiorgos, S.M. Tosh, P.J. Wood, Food Hydrocoill. 23, 949 (2009)
V. Kontogiorgos, C.G. Biliaderis, G. Kiosseoglou, G. Doxastakis, Food Hydrocoll. 18, 987 (2004)
P.J. Wood, J. Weisz, P. Fedec, V.D. Burrows, Cereal Chem. 66, 97 (1989)
P.J. Wood, J. Weisz, W. Mahn, Cereal Chem. 68, 530 (1991)
K.G. Jorgensen, Carlsberg Res. Commun. 53, 287 (1988)
A. Lazaridou, C.G. Biliaderis, M.S. Izydorczyk, Food Hydrocoll. 17, 693 (2003)
S.M. Tosh, P.J. Wood, Q. Wang, J. Weisz, Carbohydr. Polym. 55, 425 (2004)
P. Aymard, M.A.K. Williams, A.H. Clark, I.T. Norton, Langmuir 16, 7383–7391 (2000)
M. Girard, C. Sanchez, S.I. Laneuville, S.L. Turgeon, S.F. Gauthier, Colloids Surf., B Biointerfaces 35, 15 (2004)
A. Tecante, J.-L. Doublier, Carbohydr. Polym. 49, 177 (2002)
G.H. Koenderink, D.G.A.L. Aaarts, V.W.A. de Villeneuve, A.P. Philipse, R. Tuinier, H.N.W. Lekkerkerker, Biomacromolecules 4, 129 (2003)
I.T. Norton, W.J. Frith, Food Hydrocoll. 15, 543 (2001)
C. Schorsch, A.H. Clark, M.G. Jones, I.T. Norton, Colloids Surf., B Biointerfaces 12, 317 (1999)
C. Schorsch, M.G. Jones, I.T. Norton, Food Hydrocoll. 13, 89 (1999)
R.M. Musampa, M.M. Alves, J.M. Maia, Food Hydrocoll. 21, 92 (2007)
S.L. Turgeon, M. Beaulieu, C. Schmitt, C. Sanchez, Curr. Opin. Colloid Interf. Sci. 8, 401 (2003)
A. Istratov, O.F. Vyvenko, Rev. Sci. Instrum. 70, 1233 (1999)
P.W. Atkins, Physical chemistry, 4th edn. (Freeman, New York, 1990)
Q. Wang, P.J. Wood, X. Huang, W. Cui, Food Hydrocoll. 17, 845 (2003)
S. Alevisopoulos, S. Kasapis, R. Abeysekera, Carbohydr. Res. 293, 79 (1996)
R.H. Tromp, R.A.L. Jones, Macromolecules 29, 8109 (1996)
M.F. Butler, Biomacromolecules 3, 676 (2002)
J.-L. Doublier, C. Garnier, D. Renard, C. Sanchez, Curr. Opin. Colloid Interf. Sci. 5, 202 (2000)
V.Y. Grinberg, V.B. Tolstoguzov, Food Hydrocoll. 11, 145 (1997)
A. Syrbe, W.J. Bauer, H. Klostermeyer, Int. Dairy J. 8, 179 (1998)
C.G. de Kruif, R. Tuinier, Int. J. Food Sci. Technol. 34, 487 (1999)
S. Wang, J.A.P.P. van Dijk, T. Odijk, J.A.M. Smit, Biomacromolecules 2, 1080 (2001)
R. Tuinier, J.K.G. Dhont, C.G. de Kruif, Langmuir 16, 1497 (2000)
G. Zhang, E.A. Foegeding, Food Hydrocoll. 17, 785 (2003)
V.B. Tolstoguzov, Food Hydrocoll 17, 1 (2003)
V.B. Tolstoguzov, Nahrung 44, 299 (2000)
V. Kontogiorgos, H. Vaikousi, A. Lazaridou, C.G. Biliaderis, Colloids Surf. B 49, 145–152 (2006)
A. Lazaridou, C.G. Biliaderis, M. Micha-Screttas, B.R. Steele, Food Hydrocoll. 18, 837 (2004)
Acknowledgments
The authors wish to thank CreaNutrition AG (Switzerland), Swedish Oat Fiber AB (Sweden), and VINNOVA (Swedish Governmental Agency for Innovation Systems) for their generous contribution in providing financial support to this work.
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Kontogiorgos, V., Tosh, S.M. & Wood, P.J. Kinetics of Phase Separation of Oat β-Glucan/Whey Protein Isolate Binary Mixtures. Food Biophysics 4, 240–247 (2009). https://doi.org/10.1007/s11483-009-9121-z
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DOI: https://doi.org/10.1007/s11483-009-9121-z