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Nanoparticle Based Galectin-1 Gene Silencing, Implications in Methamphetamine Regulation of HIV-1 Infection in Monocyte Derived Macrophages

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Abstract

Galectin-1, an adhesion molecule, is expressed in macrophages and implicated in human immunodeficiency virus (HIV-1) viral adsorption. In this study, we investigated the effects of methamphetamine on galectin-1 production in human monocyte derived macrophages (MDM) and the role of galectin-1 in methamphetamine potentiation of HIV-1 infection. Herein we show that levels of galectin-1 gene and protein expression are significantly increased by methamphetamine. Furthermore, concomitant incubation of MDM with galectin-1 and methamphetamine facilitates HIV-1 infection compared to galectin-1 alone or methamphetamine alone. We utilized a nanotechnology approach that uses gold nanorod (GNR)-galectin-1 siRNA complexes (nanoplexes) to inhibit gene expression for galectin-1. Nanoplexes significantly silenced gene expression for galectin-1 and reversed the effects of methamphetamine on galectin-1 gene expression. Moreover, the effects of methamphetamine on HIV-1 infection were attenuated in the presence of the nanoplex in MDM.

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Acknowledgements

This work was supported by the National Institutes of Health.

K01DA024577 (JR)

R01AI085569 (SAS)

R21DA030108 (SM)

Kaleida Health Foundation (SAS)

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Innovation Center, 640 Ellicott Street, Buffalo, NY, 14203, USA

    Jessica L. Reynolds, Supriya D. Mahajan, Ravikumar Aalinkeel, Bindukumar Nair, Donald E. Sykes & Stanley A. Schwartz

  2. Department of Chemistry, Institute for Lasers and Biophotonics, University of New York at Buffalo, Natural Science Complex, Amherst, NY, 14203, USA

    Wing Cheung Law, Rui Hui & Paras N. Prasad

  3. School of Electrical and Electronic Engineering, Nanyang Technological University, Nanyang Avenue, Singapore, 639798, Singapore

    Ken-Tye Yong

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  1. Jessica L. Reynolds
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Correspondence to Jessica L. Reynolds.

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Reynolds, J.L., Law, W.C., Mahajan, S.D. et al. Nanoparticle Based Galectin-1 Gene Silencing, Implications in Methamphetamine Regulation of HIV-1 Infection in Monocyte Derived Macrophages. J Neuroimmune Pharmacol 7, 673–685 (2012). https://doi.org/10.1007/s11481-012-9379-7

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