Skip to main content
SpringerLink
Log in

A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe

  • Published:
Plasmonics Aims and scope Submit manuscript

Abstract

Nanosilver of 10-nm size was prepared by the NaBH4–sodium citrate procedure, and it was modified by a single-strand DNA (ssDNA) aptamer to fabricate an AgssDNA probe for melamine. The probe was stabile at pH 7.0 Na2HPO4–NaH2PO4 buffer solutions and in the presence of 25.0 mmol/L NaCl. Upon the addition of melamine, it interacted with the probe to aggregate big clusters, which led to the resonance scattering (RS) intensity at 470 nm increasing greatly. Under the selected conditions, the increased RS intensity (ΔI 470 nm) is linear to melamine concentration in the range of 6.31–378.4 μg/L, with a regression equation of \( \Delta {I_{{47}0{\rm{ nm}}}} = {1}.{124}c + { 10}.{8} \) and a detection limit of 3.1 μg/L. The aptamer-modified nanosilver RS assay has been applied for the determination of melamine in milk, with satisfactory results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Yu JH, Zhang CC, Dai P, Ge SG (2009) Highly selective molecular recognition and high throughput detection of melamine based on molecularly imprinted sol–gel film. Anal Chim Acta 651:209–214

    Article  CAS  Google Scholar 

  2. Wang JB, Moussa N, Hisahiro K, Koji M, Fujio K (2010) Placental transfer of melamine and its effects on rat dams and fetuses. Food Chem Toxicol 48:1791–1795

    Article  Google Scholar 

  3. Lam CW, Lan L, Che XY, Tam S, Sai S, Wong Y, Chen Y, Jin J, Tao SH, Tang XM, Yuen KY, Tam PKH (2009) Diagnosis and spectrum of melamine-related renal disease: plausible mechanism of stone formation in humans. Clin Chim Acta 402:150–155

    Article  CAS  Google Scholar 

  4. Hsieh DPH, Chiang CF, Chiang PH, Wen CP (2009) Toxicological analysis points to a lower tolerable daily intake of melamine in food. Regul Toxicol Pharmacol 55:13–16

    Article  CAS  Google Scholar 

  5. Venkatasami G, Sowa JR Jr (2010) A rapid, acetonitrile-free, HPLC method for determination of melamine in infant formula. Anal Chim Acta 665:227–230

    Article  CAS  Google Scholar 

  6. Zhang MQ, Li SJ, Yu CY, Liu GY, Jia JY, Lu C, He J, Ma YH, Zhu JM, Yu C (2010) Determination of melamine and cyanuric acid in human urine by a liquid chromatography tandem mass spectrometry. J Chromatograph B 878:758–762

    Article  CAS  Google Scholar 

  7. Xu XM, Ren YP, Zhu Y, Cai ZX, Han JL, Huang BF, Zhu Y (2009) Direct determination of melamine in dairy products by gas chromatography/mass spectrometry with coupled column separation. Anal Chim Acta 650:39–43

    Article  CAS  Google Scholar 

  8. Miao H, Fan S, Wu YN, Zhang L, Zhou PP, Li JG, Chen HJ, Zhao YF (2009) Simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography–tandem mass spectrometry. Biomed Environ Sci 22:87–94

    Article  Google Scholar 

  9. Vaclavik L, Rosmus J, Popping B, Hajslova J (2010) Rapid determination of melamine and cyanuric acid in milk powder using direct analysis in real time-time-of-flight mass spectrometry. J Chromatograph A 1217:4204–4211

    Article  CAS  Google Scholar 

  10. Tsai IL, Sun SW, Liao HW, Lin SC, Kuo CH (2010) Rapid analysis of melamine in infant formula by sweeping-micellar electrokinetic chromatography. J Chromatograph A 1216:8296–8303

    Article  Google Scholar 

  11. Cheng Y, Dong YY, Wu JH, Yang XR, Bai H, Zheng HY, Ren DM, Zou YD, Li M (2010) Screening melamine adulterant in milk powder with laser Raman spectrometry. J Food Comp Anal 23:199–202

    Article  CAS  Google Scholar 

  12. Ai KL, Liu YL, Lu LH (2009) Hydrogen-bonding recognition-induced color change of gold nanoparticles for visual detection of melamine in raw milk and infant formula. J Am Chem Soc 131:9496–9497

    Article  CAS  Google Scholar 

  13. Cao Q, Zhao H, He YJ, Li XJ, Zeng LX, Ding N, Wang J, Yang J, Wang GW (2010) Hydrogen-bonding-induced colorimetric detection of melamine by nonaggregation-based Au-NPs as a probe. Biosen Bioelectron 25:2680–2685

    Article  CAS  Google Scholar 

  14. Li L, Li BX, Cheng D, Mao LH (2010) Visual detection of melamine in raw milk using gold nanoparticles as colorimetric probe. Food Chem 122:895–900

    Article  CAS  Google Scholar 

  15. Wei F, Stacy RL, Lu CS, Ho D, Li N (2010) Rapid detection of melamine in whole milk mediated by unmodified gold nanoparticles. Appl Phys Lett 96:133702

    Article  Google Scholar 

  16. Cai W, Fan YY, Jiang ZL, Yao JE (2010) A highly sensitive and selective resonance scattering spectral assay for potassium ion based on aptamer and nanosilver aggregation reactions. Talanta 81:1810–1815

    Article  CAS  Google Scholar 

  17. Wu LP, Li YF, Huang CZ, Zhang Q (2006) Visual detection of Sudan dyes based on the plasmon resonance light scattering signals of silver nanoparticles. Anal Chem 78:5570–5577

    Article  CAS  Google Scholar 

  18. Zhang J, Jia X, Lv XJ, Deng YL, Xie HY (2010) Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells. Talanta 81:505–509

    Article  CAS  Google Scholar 

  19. Zheng J, Cheng GF, He PG, Fang YZ (2010) An aptamer-based assay for thrombin via structure switch based on gold nanoparticles and magnetic nanoparticles. Talanta 80:1868–1872

    Article  CAS  Google Scholar 

  20. Wu ZS, Zheng F, Shen GL, Yu RQ (2009) A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins. Biomaterials 30:2950–2955

    Article  CAS  Google Scholar 

  21. Jiang ZL, Wen GQ, Fan YY, Jiang CN, Liu QY, Huang Z, Liang AH (2010) A highly selective nanogold-aptamer catalytic resonance scattering spectral assay for trace Hg2+ using HAuCl4–ascorbic acid as indicator reaction. Talanta 80:1287–1291

    Article  CAS  Google Scholar 

  22. Shi C, Gu HX, Ma CP (2010) An aptamer-based fluorescent biosensor for potassium ion detection using a pyrene-labeled molecular beacon. Anal Biochem 400:99–102

    Article  CAS  Google Scholar 

  23. Wen GQ, Liang AH, Fan YY, Jiang ZL, Jiang CN (2010) A simple and rapid resonance scattering spectral method for detection of trace Hg2+ using aptamer-nanogold as probe. Plasmonics 5:1–6

    Article  CAS  Google Scholar 

  24. Chen ZG, Liu GL, Chen MH, Peng YR, Wu MY (2009) Determination of nanograms of proteins based on decreased resonance light scattering of zwitterionic gemini surfactant. Anal Biochem 384:337–342

    Article  CAS  Google Scholar 

  25. Jiang ZL, Fan YY, Chen ML, Liang AH, Liao XJ, Wen GQ, Shen XC, He XC, Pan HC, Jiang HS (2009) Resonance scattering spectral detection of trace Hg(II) using aptamer modified nanogold as probe and nanocatalyst. Anal Chem 81:5439–5445

    Article  CAS  Google Scholar 

  26. Han CP, Li HB (2010) Visual detection of melamine in infant formula at 0.1 ppm level based on silver nanoparticles. Analyst 135:583–588

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.21075023), Natural Science Foundation of Guangxi (No. 0991021Z).

Author information

Authors and Affiliations

  1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource Sciences, Guangxi Normal University, Guilin, China

    Aihui Liang, Lianping Zhou & Zhiliang Jiang

Authors
  1. Aihui Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lianping Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhiliang Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhiliang Jiang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liang, A., Zhou, L. & Jiang, Z. A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe. Plasmonics 6, 387–392 (2011). https://doi.org/10.1007/s11468-011-9215-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11468-011-9215-6

Keywords

Search

Navigation